单个磷酸盐和膦酸盐的快速分光光度法测定：其在糖磷酸盐及其膦酸盐类似物色谱分析中的应用。

A rapid spectrophotometric estimation of individual phosphates and phosphonates: its application to chromatography of sugar phosphates and their phosphonate analogues.

作者信息

Ben-Yoseph O, Sparkes M J, Dixon H B

机构信息

Department of Biochemistry, Cambridge, United Kingdom.

出版信息

Anal Biochem. 1993 Apr;210(1):195-8. doi: 10.1006/abio.1993.1172.

DOI:10.1006/abio.1993.1172

PMID:8489017

Abstract

A compound containing the -PO3H2 group (phosphoric acid, one of its monoesters, or an alkylphosphonic acid) may be rapidly assayed by the decrease it produces in the absorbance at 450 nm of a buffered acidic solution of Fe3+ and N(-3) [corrected]. The method has been used to follow chromatograms of sugar phosphates and their phosphonomethyl analogues.

摘要

含有 -PO₃H₂基团的化合物（磷酸、其单酯之一或烷基膦酸）可通过其在Fe³⁺和N(-3)的缓冲酸性溶液在450nm处吸光度的降低来快速测定[校正后]。该方法已用于跟踪糖磷酸盐及其膦酰甲基类似物的色谱图。