一种细胞色素P-450同工酶在人鼻黏膜中的免疫组织化学定位：与年龄相关的趋势
Immunohistochemical localization of a cytochrome P-450 isozyme in human nasal mucosa: age-related trends.
作者信息
Getchell M L, Chen Y, Ding X, Sparks D L, Getchell T V
机构信息
Department of Surgery, University of Kentucky College of Medicine, Lexington 40536-0084.
出版信息
Ann Otol Rhinol Laryngol. 1993 May;102(5):368-74. doi: 10.1177/000348949310200509.
Immunoperoxidase staining with an antibody to cytochrome P-450 (NMa) was used to investigate the localization of this isozyme in the human nasal mucosa. Olfactory mucosa was identified by staining of olfactory receptor cells with an antibody to olfactory marker protein. Immunoreactivity to NMa was localized in sustentacular cells in the olfactory epithelium, and in Bowman's gland acinar cells and vascular endothelial cells in the lamina propria. In the respiratory mucosa, ciliated epithelial cells, as well as serous gland acinar cells and vascular endothelial cells in the lamina propria, were immunoreactive for this isozyme. An age-related decrement in the intensity and extent of immunoreactivity within these cells was noted in nasal tissue from patients 60 years of age and over when compared with that of patients under 60 years of age. These results identify sites of xenobiotic metabolism or activation in human nasal mucosa.
采用抗细胞色素P - 450（NMa）抗体的免疫过氧化物酶染色法来研究该同工酶在人鼻黏膜中的定位。通过用抗嗅觉标记蛋白抗体对嗅觉受体细胞进行染色来识别嗅觉黏膜。对NMa的免疫反应性定位于嗅觉上皮中的支持细胞、固有层中的鲍曼腺腺泡细胞和血管内皮细胞。在呼吸黏膜中，纤毛上皮细胞以及固有层中的浆液性腺泡细胞和血管内皮细胞对该同工酶呈免疫反应性。与60岁及以下患者的鼻组织相比，60岁及以上患者鼻组织中这些细胞内免疫反应性的强度和范围存在与年龄相关的下降。这些结果确定了人鼻黏膜中异源物代谢或激活的部位。
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