Lee M Y, Clark J E, Williams D E
Toxicology Program, Oregon State University, Corvallis, Oregon 97331-6602.
Arch Biochem Biophys. 1993 May;302(2):332-6. doi: 10.1006/abbi.1993.1219.
Modulation of lung and kidney levels of flavin-containing monooxygenase (FMO) isoform B following sc administration of dexamethasone, progesterone, beta-estradiol, aldosterone, or beta-estradiol plus progesterone was examined in male rabbits. Both dexamethasone and progesterone increased FMO B protein levels in lung. N-Oxygenation of N,N'-dimethylaniline (DMA) was significantly induced in lung microsomes from rabbits treated with progesterone. In kidney, only dexamethasone was effective in inducing FMO B protein levels and DMA N-oxygenation. Surgical implantation of osmotic pumps containing beta-estradiol or progesterone into non-pregnant female rabbits resulted in elevated plasma levels of these steroids after 4-5 days at which point the lung levels of FMO B were induced by 35 and 82% for beta-estradiol and progesterone, respectively. The responsiveness of FMO B in lung to both progesterone and glucocorticoids and in kidney to only the latter is consistent with the developmental pattern of both maternal FMO B and peak plasma levels of these steroids during pregnancy.
在雄性兔子中，研究了皮下注射地塞米松、孕酮、β-雌二醇、醛固酮或β-雌二醇加孕酮后，含黄素单加氧酶（FMO）同工型B在肺和肾中的水平变化。地塞米松和孕酮均可增加肺中FMO B蛋白水平。用孕酮处理的兔子肺微粒体中，N，N'-二甲基苯胺（DMA）的N-氧化显著诱导。在肾脏中，只有地塞米松能有效诱导FMO B蛋白水平和DMA N-氧化。将含有β-雌二醇或孕酮的渗透泵手术植入未怀孕的雌性兔子体内，4-5天后这些类固醇的血浆水平升高，此时β-雌二醇和孕酮分别使肺中FMO B水平诱导升高35%和82%。肺中FMO B对孕酮和糖皮质激素均有反应，而肾脏中仅对后者有反应，这与孕期母体FMO B的发育模式以及这些类固醇的血浆峰值水平一致。
