Schafer S J, Luyten F P, Yanagishita M, Reddi A H
Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Arch Biochem Biophys. 1993 May;302(2):431-8. doi: 10.1006/abbi.1993.1236.
The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA, and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGF-AA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.
在无血清条件下，研究了重组人血小板衍生生长因子（PDGF）对牛关节软骨外植体中蛋白聚糖代谢的影响。PDGF的所有三种同工型（AB、AA和BB）均呈剂量依赖性地增加蛋白聚糖生物合成，并降低从幼年（1 - 6周）、青少年（2 - 6个月）和成年动物（2 - 3岁）获得的软骨外植体中蛋白聚糖的分解代谢速率。仅在成年组织中，PDGF - AA的生物合成刺激作用明显低于PDGF - AB。分解代谢速率的降低在较年幼的动物中更为明显。在长达12天的培养期内，三种PDGF同工型对DNA合成均无影响。蛋白聚糖单体和糖胺聚糖链的分子大小以及对软骨素酶ABC敏感的池随动物年龄的增长而减小，但不受PDGF - AB处理的影响。这些数据表明，三种重组人PDGF同工型有助于关节软骨外植体系统中的基质稳态，且无明显的促有丝分裂作用。
