用于小链霉菌合成放线菌素D的化学成分确定培养基的研制。
Development of a chemically defined medium for the synthesis of actinomycin D by Streptomyces parvulus.
作者信息
Williams W K, Katz E
出版信息
Antimicrob Agents Chemother. 1977 Feb;11(2):281-90. doi: 10.1128/AAC.11.2.281.
Abstract
A chemically defined medium, consisting of d-fructose, l-glutamic acid, l-histidine, K(2)HPO(4), MgSO(4).7H(2)O, ZnSO(4).7H(2)O, CaCl(2).2H(2)O, FeSO(4).7H(2)O, CoCl(2).6H(2)O, and deionized water, was developed for synthesis of high yields (500 to 600 mug/ml) of actinomycin D by Streptomyces parvulus. Under these nutritional conditions, growth and actinomycin formation did not follow a typical trophophase-idiophase pattern. The amino acids appeared to have a sparing action on the utilization of d-fructose which was slowly and incompletely metabolized during mycelium development and antibiotic production. A significant repression of actinomycin synthesis by S. parvulus was observed when d-glucose (0.01 to 0.25%) was added to the culture medium. The repression was not due to a decline in the pH of the medium during glucose catabolism.
摘要
一种化学成分明确的培养基，由d-果糖、l-谷氨酸、l-组氨酸、磷酸氢二钾、七水硫酸镁、七水硫酸锌、二水氯化钙、七水硫酸亚铁、六水氯化钴和去离子水组成，被开发用于由细链霉菌高产合成放线菌素D（500至600微克/毫升）。在这些营养条件下，生长和放线菌素形成并不遵循典型的营养期-静止期模式。氨基酸似乎对d-果糖的利用有节约作用，d-果糖在菌丝体发育和抗生素生产过程中代谢缓慢且不完全。当向培养基中添加d-葡萄糖（0.01%至0.25%）时，观察到细链霉菌对放线菌素合成有显著抑制作用。这种抑制不是由于葡萄糖分解代谢过程中培养基pH值的下降。
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