Finkelstein S D, Sayegh R, Bakker A, Swalsky P
Department of Pathology, Rhode Island Hospital, Brown University, Providence 02903.
Arch Surg. 1993 May;128(5):526-31; discussion 531-2. doi: 10.1001/archsurg.1993.01420170056008.
Markers that predict tumor aggressiveness on a case-by-case basis would enable individualization and optimization of oncologic therapy. To achieve this goal, the presence and specific type of K-ras-2 point mutation was determined from formalin-fixed, paraffin-embedded tissue sites in 247 primary and 166 metastatic-recurrent colorectal adenocarcinomas, using a novel approach consisting of topographic tissue selection, DNA amplification, and direct sequencing applicable to large and needle-biopsy-sized specimens. The results provide the basis for a genotypic classification of colorectal cancer capable of predicting individual tumor aggressiveness, including the pattern and extent of metastasis.
能够逐案预测肿瘤侵袭性的标志物将有助于肿瘤治疗的个体化和优化。为实现这一目标，采用一种新方法确定了247例原发性和166例转移性复发性结直肠癌福尔马林固定、石蜡包埋组织部位的K-ras-2点突变的存在情况和具体类型，该新方法包括地形组织选择、DNA扩增和适用于大标本及针吸活检大小标本的直接测序。这些结果为能够预测个体肿瘤侵袭性（包括转移模式和范围）的结直肠癌基因分型提供了依据。
