Herring J A, Shivangi U, Hall C C, Mihas A A, Lynch C, Vijay-Munshi N, Hall T J
Department of Biochemistry, Veterans Affairs Medical Center, Jackson, MS 39216, USA.
Cancer Lett. 1996 Dec 20;110(1-2):1-9. doi: 10.1016/s0304-3835(96)04447-3.
Six synchronous gastrointestinal primaries were identified in a 70 year old male with no known cancer predisposition syndrome or recognized risk factors except alcohol abuse. These specimens appeared to be independent and unrelated by gross and histopathological examination. In order to further evaluate the six tumors, we analyzed selected DNA sequences for alterations in the K-ras oncogene and p53 tumor suppressor gene. In addition, three loci were analyzed to determine microsatellite instability. Using the polymerase chain reaction, single stranded conformational polymorphism, and DNA sequencing, we demonstrated that each primary manifests genetic characteristics typical of the tissue of origin. In addition, one primary, a moderately differentiated colon adenocarcinoma, exhibited mutations not detected in the other specimens. This study suggests that these synchronous primaries arose independently and progressed along different carcinogenic pathways.
在一名70岁男性中发现了6个同时发生的胃肠道原发性肿瘤，该男性除酗酒外无已知的癌症易感综合征或公认的风险因素。通过大体和组织病理学检查，这些标本似乎是独立的且无关联。为了进一步评估这6个肿瘤，我们分析了选定的DNA序列，以检测K-ras癌基因和p53肿瘤抑制基因的改变。此外，分析了3个位点以确定微卫星不稳定性。使用聚合酶链反应、单链构象多态性和DNA测序，我们证明每个原发性肿瘤都表现出其起源组织典型的遗传特征。此外，其中一个原发性肿瘤，即中度分化的结肠腺癌，表现出在其他标本中未检测到的突变。这项研究表明，这些同时发生的原发性肿瘤是独立发生的，并沿着不同的致癌途径发展。
