Blijenberg B G, Zwang L, Heerenbrink G J
Academic Hospital Rotterdam-Dijkzigt, Department of Clinical Chemistry, The Netherlands.
Eur J Clin Chem Clin Biochem. 1993 Mar;31(3):147-51. doi: 10.1515/cclm.1993.31.3.147.
A study is reported on the determination of creatinine with the DuPont Dimension, paying special attention to interference by bilirubin. Various methods for dealing with bilirubin interference are described, including modification of the Jaffé reaction, enzymatic assay of creatinine, enzymatic destruction of bilirubin, and ultrafiltration of the serum sample. None of these modifications gave completely satisfactory results, although the Jaffé method with a ferricyanide oxidation step, and the enzymatic assay with ferrocyanide gave reasonable results with icteric specimens from adult patients, while only the latter method could be used for neonatal serum samples.
本文报道了一项关于使用杜邦Dimension测定肌酐的研究，特别关注胆红素的干扰。描述了处理胆红素干扰的各种方法，包括改良的Jaffé反应、肌酐的酶法测定、胆红素的酶促破坏以及血清样品的超滤。尽管采用铁氰化物氧化步骤的Jaffé方法和使用亚铁氰化物的酶法测定对于成年患者的黄疸标本给出了合理的结果，但这些改良方法均未得到完全令人满意的结果，而且只有后一种方法可用于新生儿血清样本。
