Boyne P, Robinson B A, Murphy P, McKay M
Clin Chem. 1985 Sep;31(9):1564-5.
We evaluated a simple, specific method for measuring plasma creatinine concentration, involving enzymatic degradation of creatinine before analysis by a kinetic Jaffé reaction. The enzymes--prepared in a dry, stable form--are convenient to use and eliminate the need to correct for sample dilution. As little as 50 microL of plasma is required, making the procedure ideal for use with samples from children. Between-day precision studies for determination of plasma creatinine at concentrations of 70 and 470 mumol/L yielded CVs of 7.8 and 4.3%, respectively. Analytical recovery of creatinine added to plasma samples ranged from 94 to 113%. Linear regression analysis demonstrated excellent agreement between results by this method and those by a "high performance" liquid-chromatographic technique.
我们评估了一种简单、特异的测量血浆肌酐浓度的方法，该方法在通过动力学Jaffé反应进行分析之前对肌酐进行酶促降解。这些以干燥、稳定形式制备的酶使用方便，无需对样品稀释进行校正。仅需50微升血浆，该方法非常适合用于儿童样本。对浓度为70和470μmol/L的血浆肌酐进行日间精密度研究，变异系数（CV）分别为7.8%和4.3%。添加到血浆样本中的肌酐的分析回收率在94%至113%之间。线性回归分析表明，该方法的结果与“高效”液相色谱技术的结果高度一致。
