正常及链脲佐菌素诱导的糖尿病仓鼠肾脏的凝集素组织化学研究

Lectin histochemical study on the kidney of normal and streptozotocin-induced diabetic hamsters.

作者信息

Aguirre J I, Han J S, Itagaki S, Doi K

机构信息

Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

Histol Histopathol. 1993 Apr;8(2):273-8.

PMID:8490254

Abstract

Lectin histochemical characteristics of the kidney of normal and streptozotocin (SZ)-induced diabetic APA hamsters were investigated. Paraffin sections of the kidney of animals killed at 1 and 3 months after SZ-injection were stained with the following 10 lectins: PNA, BPA, DBA, SBA, GSA-I, GSA-II, MPA, WGA, UEA-1 and Con A. Renal lectin binding characteristics of normal APA hamsters differed in some aspects from those in other species reported previously. In the kidney of SZ-induced diabetic APA hamsters, binding activities of some lectins increased in the affected Bowman's capsules and glomeruli as well as in degenerated epithelial cells of uriniferous tubules. Among them, GSA-II in particular exhibited strong binding activity to the degenerated epithelial cells of Bowman's capsules, distal convoluted tubules and collecting ducts.

摘要

研究了正常和链脲佐菌素（SZ）诱导的糖尿病APA仓鼠肾脏的凝集素组织化学特征。对注射SZ后1个月和3个月处死动物的肾脏石蜡切片用以下10种凝集素进行染色：花生凝集素（PNA）、双花扁豆凝集素（BPA）、荆豆凝集素（DBA）、大豆凝集素（SBA）、麦芽凝集素I（GSA-I）、麦芽凝集素II（GSA-II）、桑寄生凝集素（MPA）、小麦胚凝集素（WGA）、荆豆凝集素I（UEA-1）和刀豆球蛋白A（Con A）。正常APA仓鼠的肾脏凝集素结合特征在某些方面与先前报道的其他物种不同。在SZ诱导的糖尿病APA仓鼠的肾脏中，一些凝集素在受影响的肾小囊和肾小球以及肾小管变性上皮细胞中的结合活性增加。其中，GSA-II尤其对肾小囊、远曲小管和集合管的变性上皮细胞表现出强烈的结合活性。