Nishida S, Hosokawa K, Kusunoki T, Koezuka M, Akai F, Nakano T, Funasaka K, Yoshioka Y, Murata K, Hashimoto S
Department of Biochemistry and Oncology, Kinki University School of Medicine, Osaka, Japan.
Histol Histopathol. 1993 Apr;8(2):329-37.
Using normal human thyroid cells and tumor cells, the reconstruction of various diseased cells in collagen gel as well as the relationship between the morphology of colonies in collagen-embedded culture and the biological behavior (benignity, malignancy, metastasis, and invasion) of the original tumors were studied. In collagen gel culture, normal thyroid cells reorganized follicle-like constructions, and follicular adenoma cells showed in vivo-like constructions. However, two different types of colonies were observed in cultures of cells from papillary carcinomas. One was the branching type with many outgrowths projecting to three dimensions and the other was the spherical type without any outgrowths. These spherical colonies were observed in all cases of papillary carcinoma, but varied from one case to another. Metastasis and invasion were detected during pathological examination in cases with a high ratio of spherical colonies. Our results indicate that cells from highly metastatic and invasive thyroid cancer form spherical colonies in the collagen gel culture, and that this collagen culture is a useful method for studying the heterogeneity of tumor cells as well as the metastasis and invasive ability of tumor cells in vitro.
利用正常人甲状腺细胞和肿瘤细胞，研究了胶原凝胶中各种病变细胞的重建，以及胶原包埋培养中集落形态与原发肿瘤生物学行为（良性、恶性、转移和侵袭）之间的关系。在胶原凝胶培养中，正常甲状腺细胞重组形成滤泡样结构，滤泡性腺瘤细胞呈现出类似体内的结构。然而，在乳头状癌细胞培养中观察到两种不同类型的集落。一种是分支型，有许多向三维空间突出的生长物；另一种是无任何生长物的球形集落。所有乳头状癌病例均观察到这些球形集落，但不同病例有所差异。在球形集落比例高的病例中，病理检查发现有转移和侵袭。我们的结果表明，高转移性和侵袭性甲状腺癌细胞在胶原凝胶培养中形成球形集落，并且这种胶原培养是研究肿瘤细胞异质性以及肿瘤细胞体外转移和侵袭能力的有用方法。
