Kusunoki T, Yoshioka Y, Kohda Y, Murata K, Nishida S, Tomura T, Koezuka M, Ohta F
Department of Otolaryngology, Kinki University School of Medicine, Osaka.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Jan;96(1):35-40. doi: 10.3950/jibiinkoka.96.35.
Thyroid cells from 14 normal subjects, two patients with Grave's disease, four patients with follicular adenoma and eight patients with papillary carcinoma were cultured in collagen gel. The colonies of these cells were stereoscopically observed and their morphological characteristics were studied with regard to relation with pathological findings of mother tumor, extra-capsular invasion and metastatic potential. For normal thyroid, Grave's disease and follicular adenoma (except for one case), their own characteristic branching type colonies were found. For papillary carcinoma, both branching type and spheroid type of colonies were observed. The ratio of branching type/spheroid type varied individually in the patients with papillary carcinoma. However, the spheroid type was found to trend to be predominant in patients with extra-capsular invasion and/or lymph node metastasis. This means that the observation of spheroid type colonies in collagen gel is suggestive of risk of extra-capsular invasion or lymph node metastasis. From the obtained results, it seemed possible to diagnose poorly-differentiated cells in vitro by morphologically observing colonies of human thyroid papillary carcinoma cells developing in collagen gel.
将来自14名正常受试者、2名格雷夫斯病患者、4名滤泡性腺瘤患者和8名乳头状癌患者的甲状腺细胞培养于胶原凝胶中。对这些细胞的集落进行立体观察，并研究其形态学特征与母瘤病理结果、包膜外侵犯及转移潜能的关系。对于正常甲状腺、格雷夫斯病和滤泡性腺瘤（除1例），发现了它们各自特征性的分支型集落。对于乳头状癌，观察到了分支型和球型集落。在乳头状癌患者中，分支型/球型的比例因人而异。然而，在有包膜外侵犯和/或淋巴结转移的患者中，球型集落倾向于占主导。这意味着在胶原凝胶中观察到球型集落提示有包膜外侵犯或淋巴结转移的风险。根据所得结果，通过形态学观察在胶原凝胶中生长的人甲状腺乳头状癌细胞集落，似乎有可能在体外诊断低分化细胞。
