HLA - A基因座周围七个新基因的定位

Localization of seven new genes around the HLA-A locus.

作者信息

el Kahloun A, Chauvel B, Mauvieux V, Dorval I, Jouanolle A M, Gicquel I, Le Gall J Y, David V

机构信息

Laboratoire de Biochimie Médicale B, Faculté de Médecine, Rennes, France.

出版信息

Hum Mol Genet. 1993 Jan;2(1):55-60. doi: 10.1093/hmg/2.1.55.

DOI:10.1093/hmg/2.1.55

PMID:8490624

Abstract

A yeast artificial chromosome (YAC B30) with a 320 kb insert of genomic DNA which includes the HLA-A gene was used to screen a cDNA library of human duodenal mucosa. Seven cDNA clones were isolated which correspond to seven new non-HLA class I structural genes. These new genes are located within a region that may well contain the gene responsible for hemochromatosis and have therefore been named HCG I-VII (Hemochromatosis Candidate Gene). HCG I, III, V and VI are probably single copy genes, situated at 180, 155, 140 and 230 kb centromeric to HLA-A, respectively. HCG II, IV and VII present several copies: one copy of HCG II, one of HCG IV and one of HCG VII are centromeric to HLA-A (at 30, 70 and 100 kb respectively). Another copy of HCG IV is 20 kb telomeric to HLA-A. Each of the genes localized on the YAC B30 is associated with an CpG/HTF island.

摘要

一个带有包含HLA - A基因的320 kb基因组DNA插入片段的酵母人工染色体（YAC B30）被用于筛选人十二指肠黏膜的cDNA文库。分离出了七个cDNA克隆，它们对应于七个新的非HLA I类结构基因。这些新基因位于一个很可能包含血色素沉着症相关基因的区域内，因此被命名为HCG I - VII（血色素沉着症候选基因）。HCG I、III、V和VI可能是单拷贝基因，分别位于HLA - A着丝粒方向180、155、140和230 kb处。HCG II、IV和VII有多个拷贝：HCG II的一个拷贝、HCG IV的一个拷贝和HCG VII的一个拷贝位于HLA - A着丝粒方向（分别在30、70和100 kb处）。HCG IV的另一个拷贝位于HLA - A端粒方向20 kb处。定位在YAC B30上的每个基因都与一个CpG/HTF岛相关联。