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通过cDNA杂交筛选从血色素沉着病区域（6p21.3）分离新型非HLA基因片段。

Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection.

作者信息

Goei V L, Parimoo S, Capossela A, Chu T W, Gruen J R

机构信息

Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510.

出版信息

Am J Hum Genet. 1994 Feb;54(2):244-51.

PMID:8304341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1918154/
Abstract

It has previously been shown that cDNA hybridization selection can identify and recover novel genes from large cloned genomic DNA such as cosmids or YACs. In an effort to identify candidate genes for hemochromatosis, this technique was applied to a 320-kb YAC containing the HLA-A gene. A short fragment cDNA library derived from human duodenum was selected with the YAC DNA. Ten novel gene fragments were isolated, characterized, and localized on the physical map of the YAC.

摘要

先前已经表明，cDNA杂交筛选能够从诸如黏粒或酵母人工染色体（YAC）等大型克隆基因组DNA中鉴定并回收新基因。为了鉴定血色素沉着症的候选基因，该技术被应用于一个包含HLA - A基因的320 kb酵母人工染色体。用人十二指肠来源的短片段cDNA文库与该酵母人工染色体DNA进行筛选。分离出10个新的基因片段，对其进行了表征，并定位在酵母人工染色体的物理图谱上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9448/1918154/c3769d9ec898/ajhg00047-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9448/1918154/f3bb35a5a336/ajhg00047-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9448/1918154/c3769d9ec898/ajhg00047-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9448/1918154/f3bb35a5a336/ajhg00047-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9448/1918154/c3769d9ec898/ajhg00047-0091-a.jpg

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本文引用的文献

1
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Hum Mol Genet. 1993 Jan;2(1):55-60. doi: 10.1093/hmg/2.1.55.
2
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.人α-、β-和γ-肌动蛋白mRNA全长cDNA克隆的分离与鉴定：骨骼肌肌动蛋白而非细胞质肌动蛋白具有一个随后会被去除的氨基端半胱氨酸。
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Immunogenetics. 1996;43(4):175-81. doi: 10.1007/BF00587297.
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International workshop on molecular genetics of haemochromatosis, held at Villa Feltrinelli, Gargnano (Bs), Italy, 25 September 1994.血色素沉着症分子遗传学国际研讨会，于1994年9月25日在意大利加尔尼亚诺（布雷西亚省）的费尔特里内利别墅举行。
J Med Genet. 1995 Apr;32(4):320-3. doi: 10.1136/jmg.32.4.320.
人类重复DNA序列、非α-珠蛋白基因和主要组织相容性位点基因。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1079-86.
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Carrier detection of Hemophilia B by using a restriction site polymorphism associated with the coagulation Factor IX gene.利用与凝血因子IX基因相关的限制性酶切位点多态性进行B型血友病的携带者检测。
J Clin Invest. 1984 May;73(5):1491-5. doi: 10.1172/JCI111354.
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Survival and causes of death in cirrhotic and in noncirrhotic patients with primary hemochromatosis.原发性血色素沉着症的肝硬化和非肝硬化患者的生存率及死亡原因
N Engl J Med. 1985 Nov 14;313(20):1256-62. doi: 10.1056/NEJM198511143132004.
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Removal of repeated sequences from hybridisation probes.从杂交探针中去除重复序列。
Nucleic Acids Res. 1985 Mar 25;13(6):1905-22. doi: 10.1093/nar/13.6.1905.
7
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Identification of multiple HTF-island associated genes in the human major histocompatibility complex class III region.
EMBO J. 1989 Aug;8(8):2305-12. doi: 10.1002/j.1460-2075.1989.tb08357.x.
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Differential recognition by human cytotoxic T cell clones of human M1 fibroblasts transfected with an HLA-B7 gene (JY150) suggests the existence of two different HLA-B7 alleles in the cell line JY (HLA-A2,2;B7,7;Cw-,-;DR4,w6).人细胞毒性T细胞克隆对转染了HLA - B7基因的人M1成纤维细胞（JY150）的差异识别表明，细胞系JY（HLA - A2,2；B7,7；Cw -,-；DR4,w6）中存在两种不同的HLA - B7等位基因。
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