Senba E, Matsunaga K, Tohyama M, Noguchi K
Department of Anatomy and Neurobiology, Wakayama Medical College, Japan.
Brain Res Bull. 1993;31(3-4):329-44. doi: 10.1016/0361-9230(93)90225-z.
To clarify which brain regions are activated by stress, we used expression of the proto-oncogene, c-fos, as a marker. An increased number of neurons expressing Fos-like immunoreactivity in their nuclei was observed in discrete brain regions, such as the lateral septum, midline nuclei of the thalamus, paraventricular hypothalamic nucleus, brain stem catecholaminergic, and serotonergic neurons, in response to pain or immobilization stress. Distribution patterns of Fos-like immunoreactive neurons were quite similar in animals subjected to pain or immobilization. Whether or not neurons projecting to the spinal cord to activate the sympathetic pathway express Fos-like immunoreactivity was examined by means of fluorescent double-labeling using fluoro-gold (FG) as a tracer. In the PVH, Fos-immunoreactive neurons were localized in the dorsal medial parvocellular part, although those projecting to the spinal cord were localized dorsally and ventrally. Less than 1% of Fos-positive neurons in this nucleus was colocalized with FG. Among fos-like immunoreactive catecholaminergic and serotonergic neurons in the brain stem, those in the A5 region and raphe pallidus projected to the spinal cord.
为了明确应激激活哪些脑区，我们使用原癌基因c-fos的表达作为标志物。在离散的脑区，如外侧隔、丘脑中线核、室旁下丘脑核、脑干儿茶酚胺能神经元和5-羟色胺能神经元中，观察到因疼痛或制动应激而导致细胞核中表达Fos样免疫反应性的神经元数量增加。在遭受疼痛或制动的动物中，Fos样免疫反应性神经元的分布模式非常相似。通过使用荧光金（FG）作为示踪剂的荧光双标记法，研究了投射到脊髓以激活交感神经通路的神经元是否表达Fos样免疫反应性。在室旁核中，Fos免疫反应性神经元定位于背内侧小细胞部，而投射到脊髓的神经元则定位于背侧和腹侧。该核中不到1%的Fos阳性神经元与FG共定位。在脑干中表达Fos样免疫反应性的儿茶酚胺能和5-羟色胺能神经元中，A5区和中缝苍白核中的神经元投射到脊髓。
