Shibuya M, Ito S, Davis R L, Wilson C B, Hoshino T
Department of Neurological Surgery, School of Medicine, University of California, San Francisco.
Cancer. 1993 May 15;71(10):3109-13. doi: 10.1002/1097-0142(19930515)71:10<3109::aid-cncr2820711035>3.0.co;2-f.
Cell kinetics studies performed with immunohistochemical techniques to estimate the S-phase fraction have elucidated the proliferative potential of individual brain tumors.
The authors developed a new double-labeling method that enables other cell kinetics variables, including the duration of the S-phase (Ts) and the potential doubling time (Tp), to be measured from a single biopsy specimen. Using this method, 100 brain tumors were labeled with bromodeoxyuridine (BUdR) in situ and with iododeoxyuridine in vitro; labeled cells were identified by double staining with immunogold-silver and alkaline phosphatase techniques.
Ts was fairly uniform (mean, 9.2 +/- 2.1 hour [+/- standard deviation]); range, 6.0-13.7 hours), but Tp varied from 1 day to more than 2 months. The Tp values correlated closely with the BUdR labeling index (LI), or S-phase fraction, and can be calculated from the equation: Tp = 26.9/LI1.02 (r = 0.98, P < 0.005).
This new method facilitates the quantitation of the proliferative potential of individual brain tumors. The S-phase fraction, Ts, and Tp can be calculated from analysis of a single biopsy specimen. This method can be used to estimate the prognosis of individual patients with brain tumors and to select treatment modalities more directly than is possible with single-labeling studies with BUdR.
运用免疫组化技术进行细胞动力学研究以评估S期细胞比例，已阐明了个体脑肿瘤的增殖潜能。
作者研发了一种新的双标记方法，该方法能够从单个活检标本中测量包括S期持续时间（Ts）和潜在倍增时间（Tp）在内的其他细胞动力学变量。使用此方法，对100例脑肿瘤进行原位溴脱氧尿苷（BUdR）标记和体外碘脱氧尿苷标记；通过免疫金银染色和碱性磷酸酶技术双重染色鉴定标记细胞。
Ts相当一致（平均值为9.2±2.1小时[±标准差]；范围为6.0 - 13.7小时），但Tp从1天到超过2个月不等。Tp值与BUdR标记指数（LI）或S期细胞比例密切相关，可通过以下公式计算：Tp = 26.9/LI1.02（r = 0.98，P < 0.005）。
这种新方法有助于对个体脑肿瘤的增殖潜能进行定量分析。S期细胞比例、Ts和Tp可通过对单个活检标本的分析来计算。与使用BUdR的单标记研究相比，该方法可更直接地用于评估个体脑肿瘤患者的预后并选择治疗方式。
