Nakajima M, Nakasu S, Morikawa S, Inubushi T
Department of Neurosurgery, Shiga University of Medical Science, Japan.
Acta Neurochir (Wien). 1998;140(6):607-12; discussion 612-3. doi: 10.1007/s007010050148.
We estimated the volume doubling time (Vd) of the ethyl-nitrosourea-induced rat glioma by serial magnetic resonance imaging, and the results were compared with potential doubling time (Tp) determined immunohistochemically. Vd ranged from 3.3 to 29.2 days (11.3 +/- 7.74) and Tp ranged from 2.3 to 13.3 days (6.81 +/- 3.33). Each tumour showed a wide range of bromodeoxyuridine (BUdR) labelling indices (LI), however, Vd and Tp correlated well with BUdR-LI. Vd was estimated as 17.6 x BUdR-LI-0.63 (R = -0.76, P < 0.001, n = 13) and Tp was estimated as 22.6 x BUdR-LI-1.02 (R = -0.92, P < 0.0001, n = 12). In addition, we compared the apoptotic indices (AI), determined by terminal deoxynucleotidyltransferase (Tdt)-mediated biotinylated dUTP-biotin nick-end labelling (TUNEL) techniques, with BUdR-LI and mitoses indices (MI). The results were: AI = 0.23 + 0.25Ln(BUdR-LI) (R = 0.971, n = 8, P < 0.0001) and AI = 1.05 + 0.29Ln(MI) (R = 0.937, n = 8, P < 0.001). Cell loss factors (CLF) also correlated well with BUdR-LI and MI. However, CLF calculated from Tp and Vd were lower than the values previously presumed, probably because of shorter Vd than true doubling time for tumour cell population. These results suggest that even malignant tumours retain a mechanism of adjusting their growth at least partly.
我们通过连续磁共振成像估计了乙基亚硝基脲诱导的大鼠胶质瘤的体积倍增时间（Vd），并将结果与免疫组织化学测定的潜在倍增时间（Tp）进行比较。Vd范围为3.3至29.2天（11.3±7.74），Tp范围为2.3至13.3天（6.81±3.33）。每个肿瘤的溴脱氧尿苷（BUdR）标记指数（LI）范围广泛，然而，Vd和Tp与BUdR-LI密切相关。Vd估计为17.6×BUdR-LI-0.63（R = -0.76，P < 0.001，n = 13），Tp估计为22.6×BUdR-LI-1.02（R = -0.92，P < 0.0001，n = 12）。此外，我们将通过末端脱氧核苷酸转移酶（Tdt）介导的生物素化dUTP-生物素缺口末端标记（TUNEL）技术测定的凋亡指数（AI）与BUdR-LI和有丝分裂指数（MI）进行比较。结果为：AI = 0.23 + 0.25Ln（BUdR-LI）（R = 0.971，n = 8，P < 0.0001）和AI = 1.05 + 0.29Ln（MI）（R = 0.937，n = 8，P < 0.001）。细胞丢失因子（CLF）也与BUdR-LI和MI密切相关。然而，根据Tp和Vd计算的CLF低于先前推测的值，可能是因为Vd比肿瘤细胞群体的真正倍增时间短。这些结果表明，即使是恶性肿瘤也至少部分保留了调节其生长的机制。
