Seim H, Aurich H, Strack E
Arch Microbiol. 1977 Mar 1;112(2):201-6. doi: 10.1007/BF00429336.
The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain grew only on L-carnitine, L-O-acylcarnitines, and gamma-butyrobetaine as the sole carbon sources. The utilization of these compounds and the growth correlated with the cleavage of the C-N bond and thereby with the formation of trimethylamin. D-Carnitine was metabolized, if an additional carbon source, like L-carnitine, was present in the incubation mixture, or if the bacteria were preincubated with L- or DL-carnitine, but no growth was observed on D-carnitine as the sole carbon source. The bacteria oxidized choline to glycinebetaine in the presence of additional carbon sources, glycinebetaine itself was not assimilated. With regard to the catabolism of quaternary nitrogen compounds Acinetobacter calcoaceticus shows a different pathway in comparison with other bacterial species metabolizing carnitine.
通过生长控制和代谢产物的定量检测，研究了醋酸钙不动杆菌对肉碱及其衍生物（O-酰基肉碱、肉碱羧基衍生物）以及结构相关的三甲铵化合物（甜菜碱和含氮碱）的利用情况。该菌株仅能以L-肉碱、L-O-酰基肉碱和γ-丁酸甜菜碱作为唯一碳源生长。这些化合物的利用和生长与C-N键的断裂相关，进而与三甲胺的形成相关。如果在培养混合物中存在额外的碳源，如L-肉碱，或者细菌预先用L-或DL-肉碱进行预培养，D-肉碱可以被代谢，但以D-肉碱作为唯一碳源时未观察到生长。在存在额外碳源的情况下，细菌将胆碱氧化为甘氨酸甜菜碱，甘氨酸甜菜碱本身不能被同化。关于季铵化合物的分解代谢，与其他代谢肉碱的细菌物种相比，醋酸钙不动杆菌表现出不同的途径。
