海星的卵母细胞成熟是由一种G蛋白的βγ亚基复合物介导的。
Oocyte maturation in starfish is mediated by the beta gamma-subunit complex of a G-protein.
作者信息
Jaffe L A, Gallo C J, Lee R H, Ho Y K, Jones T L
机构信息
Physiology Department, University of Connecticut Health Center, Farmington 06032.
出版信息
J Cell Biol. 1993 May;121(4):775-83. doi: 10.1083/jcb.121.4.775.
Abstract
The stimulation of meiotic maturation of starfish oocytes by the hormone 1-methyladenine is mimicked by injection of beta gamma subunits of G-proteins from either retina or brain. Conversely, the hormone response is inhibited by injection of the GDP-bound forms of alpha i1 or alpha t subunits, or by injection of phosducin; all of these proteins should bind free beta gamma. alpha-subunit forms with reduced affinity for beta gamma (alpha i1 or alpha t bound to hydrolysis-resistant GTP analogs, or alpha i1-GMPPCP treated with trypsin to remove the amino terminus of the protein) are less effective inhibitors of 1-methyladenine action. These results indicate that the beta gamma subunit of a G-protein mediates 1-methyladenine stimulation of oocyte maturation.
摘要
通过注射来自视网膜或大脑的G蛋白βγ亚基，可模拟激素1 - 甲基腺嘌呤对海星卵母细胞减数分裂成熟的刺激作用。相反，注射与GDP结合的αi1或αt亚基形式，或注射磷光蛋白可抑制激素反应；所有这些蛋白质都应结合游离的βγ。对βγ亲和力降低的α亚基形式（与抗水解GTP类似物结合的αi1或αt，或用胰蛋白酶处理以去除蛋白质氨基末端的αi1 - GMPPCP）对1 - 甲基腺嘌呤作用的抑制效果较差。这些结果表明，G蛋白的βγ亚基介导了1 - 甲基腺嘌呤对卵母细胞成熟的刺激作用。
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