使用肝素亲和柱通过快速蛋白质液相色谱法将高密度脂蛋白分离为载脂蛋白E含量低和载脂蛋白E含量高的亚组分。

Separation of high-density lipoproteins into apolipoprotein E-poor and apolipoprotein E-rich subfractions by fast protein liquid chromatography using a heparin affinity column.

作者信息

O'Brien T, Buithieu J, Nguyen T T, Klein L, Bren N, Wentworth M, Hallaway B J, Kottke B A

机构信息

Atherosclerosis Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905.

出版信息

J Chromatogr. 1993 Apr 2;613(2):239-46. doi: 10.1016/0378-4347(93)80138-t.

DOI:10.1016/0378-4347(93)80138-t

PMID:8491809

Abstract

The aim of this paper is to describe a new methodology for the separation of human high-density lipoproteins (HDL) into apolipoprotein (apo) E-poor and apo E-rich subfractions by fast protein liquid chromatography (FPLC) using a heparin affinity column. Recoveries for apolipoproteins AI, AII, CI, CII, CIII, and E were 68.9, 74.7, 71.9, 73.5, 40.0, and 55.8%, respectively. We provide suggestive evidence that apo E-rich HDL is produced from apo E-poor HDL by the displacement of apo AI by apo E. Apo E-poor HDL was the predominant fraction. The molar ratio of apo E to apo AI in apo E-poor HDL was 0.02 and 0.01 for the subjects studied while in apo E-rich HDL it was 1.86 and 1.25. The molar ratios of the C apolipoproteins to apo AI are markedly different between the subfractions.

摘要

本文旨在描述一种新方法，即使用肝素亲和柱通过快速蛋白质液相色谱法（FPLC）将人类高密度脂蛋白（HDL）分离为载脂蛋白（apo）E含量低和载脂蛋白E含量高的亚组分。载脂蛋白AI、AII、CI、CII、CIII和E的回收率分别为68.9%、74.7%、71.9%、73.5%、40.0%和55.8%。我们提供了提示性证据，表明载脂蛋白E含量高的HDL是由载脂蛋白E取代载脂蛋白AI从载脂蛋白E含量低的HDL产生的。载脂蛋白E含量低的HDL是主要组分。在所研究的受试者中，载脂蛋白E含量低的HDL中载脂蛋白E与载脂蛋白AI的摩尔比为0.02和0.01，而在载脂蛋白E含量高的HDL中该摩尔比为1.86和1.25。各亚组分中C载脂蛋白与载脂蛋白AI的摩尔比明显不同。