O'Brien T, Buithieu J, Nguyen T T, Klein L, Bren N, Wentworth M, Hallaway B J, Kottke B A
Atherosclerosis Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905.
J Chromatogr. 1993 Apr 2;613(2):239-46. doi: 10.1016/0378-4347(93)80138-t.
The aim of this paper is to describe a new methodology for the separation of human high-density lipoproteins (HDL) into apolipoprotein (apo) E-poor and apo E-rich subfractions by fast protein liquid chromatography (FPLC) using a heparin affinity column. Recoveries for apolipoproteins AI, AII, CI, CII, CIII, and E were 68.9, 74.7, 71.9, 73.5, 40.0, and 55.8%, respectively. We provide suggestive evidence that apo E-rich HDL is produced from apo E-poor HDL by the displacement of apo AI by apo E. Apo E-poor HDL was the predominant fraction. The molar ratio of apo E to apo AI in apo E-poor HDL was 0.02 and 0.01 for the subjects studied while in apo E-rich HDL it was 1.86 and 1.25. The molar ratios of the C apolipoproteins to apo AI are markedly different between the subfractions.
本文旨在描述一种新方法，即使用肝素亲和柱通过快速蛋白质液相色谱法（FPLC）将人类高密度脂蛋白（HDL）分离为载脂蛋白（apo）E含量低和载脂蛋白E含量高的亚组分。载脂蛋白AI、AII、CI、CII、CIII和E的回收率分别为68.9%、74.7%、71.9%、73.5%、40.0%和55.8%。我们提供了提示性证据，表明载脂蛋白E含量高的HDL是由载脂蛋白E取代载脂蛋白AI从载脂蛋白E含量低的HDL产生的。载脂蛋白E含量低的HDL是主要组分。在所研究的受试者中，载脂蛋白E含量低的HDL中载脂蛋白E与载脂蛋白AI的摩尔比为0.02和0.01，而在载脂蛋白E含量高的HDL中该摩尔比为1.86和1.25。各亚组分中C载脂蛋白与载脂蛋白AI的摩尔比明显不同。
