使用ASPEC系统结合Clean Screen DAU柱进行生物流体中药物筛查的半自动固相萃取程序。

Semi-automated solid-phase extraction procedure for drug screening in biological fluids using the ASPEC system in combination with Clean Screen DAU columns.

作者信息

Chen X H, Franke J P, Ensing K, Wijsbeek J, de Zeeuw R A

机构信息

University Centre for Pharmacy, Department of Analytical Chemistry and Toxicology, Groningen, Netherlands.

出版信息

J Chromatogr. 1993 Apr 2;613(2):289-94. doi: 10.1016/0378-4347(93)80144-s.

DOI:10.1016/0378-4347(93)80144-s

PMID:8491815

Abstract

The use of a semi-automated solid-phase extraction system (ASPEC) for the screening of drugs in plasma and urine on a single mixed-mode column (Clean Screen DAU) is described. The processes of column preconditioning, sample application, column wash, pH adjustment and elution of the drugs were accomplished by the ASPEC. After off-line evaporation, the residues were injected into a wide-bore capillary gas chromatograph. The recoveries of the tested drugs were in the range of 73-96%, with relative standard deviations less than 5% at a concentration level of 2 micrograms/ml.

摘要

描述了使用半自动固相萃取系统（ASPEC）在单一混合模式柱（Clean Screen DAU）上对血浆和尿液中的药物进行筛查的方法。柱预处理、样品上样、柱冲洗、pH调节和药物洗脱等过程均由ASPEC完成。离线蒸发后，将残留物注入宽口径毛细管气相色谱仪。被测药物的回收率在73%至96%之间，在浓度为2微克/毫升时相对标准偏差小于5%。