Oertel R, Richter K, Gramatté T, Kirch W
Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Germany.
J Chromatogr A. 1998 Feb 27;797(1-2):203-9. doi: 10.1016/s0021-9673(97)00966-7.
An automated two column HPLC system with the new packing material LiChrospher RP-18 ADS (alkyl-diol-silica) was tested for the determination of several drugs and metabolites (talinolol, celiprolol, metoprolol, oxprenolol, triamterene, trimethoprim, tiracizine, articaine, detajmium, ajmaline, lamotrigine) in various biological fluids (serum, urine, intestinal aspirates, supernatants of cell cultures and supernatants after protein denaturation). The method allows the direct injection of biological fluids into a reversed-phase HPLC system and on-line clean-up and sample enrichment by a column-switching technique. Precision, accuracy and sensitivity were similar to conventional assays as described in the literature. With this new method it was possible to measure drug concentrations in various biological fluids without changing the sample preparation procedure. In some cases an additional sample preparation like protein denaturation or solid-phase extraction was advantageous to enhance the sensitivity of the method and the life-time of the ADS column.
使用配备新型填充材料LiChrospher RP - 18 ADS（烷基二醇硅胶）的自动两柱高效液相色谱系统，对多种生物流体（血清、尿液、肠道吸出物、细胞培养上清液和蛋白质变性后的上清液）中的几种药物和代谢物（他林洛尔、塞利洛尔、美托洛尔、氧烯洛尔、氨苯蝶啶、甲氧苄啶、曲西拉嗪、阿替卡因、地他铵、阿马林、拉莫三嗪）进行了测定。该方法允许将生物流体直接注入反相高效液相色谱系统，并通过柱切换技术进行在线净化和样品富集。精密度、准确度和灵敏度与文献中描述的传统分析方法相似。采用这种新方法，可以在不改变样品制备程序的情况下测量各种生物流体中的药物浓度。在某些情况下，额外的样品制备，如蛋白质变性或固相萃取，有利于提高方法的灵敏度和ADS柱的使用寿命。
