Pan F M, Chang W C
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
J Chromatogr. 1993 Apr 2;613(2):326-9. doi: 10.1016/0378-4347(93)80149-x.
Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.
采用径向Pak C18柱进行反相高效液相色谱（HPLC），从哺乳动物、鸟类、两栖动物和鱼类的垂体中纯化多种生长激素（GH）蛋白。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，从垂体中回收的GH占总蛋白的比例高达0.43%，且具有高度的同质性。经HPLC纯化的GH在琼脂凝胶上进行免疫扩散研究时显示出相同或近乎相同的反应。该方法为分析或制备规模的脊椎动物GH提供了一种方便快捷的纯化方法。
