烟草花叶病毒外壳蛋白单克隆抗体对体外共翻译时病毒解体的抑制作用。
Inhibition of in vitro cotranslational disassembly of tobacco mosaic virus by monoclonal antibodies to the viral coat protein.
作者信息
Saunal H, Witz J, Van Regenmortel M H
机构信息
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
出版信息
J Gen Virol. 1993 May;74 ( Pt 5):897-900. doi: 10.1099/0022-1317-74-5-897.
It has been shown by others that translation of tobacco mosaic virus (TMV) RNA may begin before uncoating of particles is complete. We provide evidence that this cotranslational disassembly can be inhibited by incubating TMV treated at pH 8 with certain monoclonal antibodies (MAbs) specific for TMV coat protein. The most efficient inhibition was achieved by incubation with some anti-metatope MAbs known to bind to the TMV extremity that becomes disassembled first and contains the 5' end of the RNA, as well as with some anti-cryptotope MAbs that bind only to dissociated coat protein subunits.
其他人已表明，烟草花叶病毒（TMV）RNA的翻译可能在病毒粒子脱壳完成之前就开始了。我们提供的证据表明，通过将在pH 8条件下处理过的TMV与某些针对TMV衣壳蛋白的单克隆抗体（MAb）一起孵育，可以抑制这种共翻译解聚。通过与一些已知能结合TMV最先解聚且含有RNA 5'端的末端的抗元位MAb以及一些仅与解离的衣壳蛋白亚基结合的抗隐位MAb一起孵育，可实现最有效的抑制。
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