Rooney P, Walker D, Grant M E, McClure J
Department of Pathological Sciences and Biochemistry, University of Manchester, U.K.
J Pathol. 1993 Mar;169(3):375-81. doi: 10.1002/path.1711690315.
Rodent Achilles tendons were subjected to midpoint tenotomy and allowed to recover for various times in situ before the operated tissue was removed, placed into a Millipore diffusion chamber, and inserted intraperitoneally into syngeneic hosts. Diffusion chambers were then removed at weekly intervals, such that the total time after the operation (i.e., time allowed to recover in situ plus time within the diffusion chamber) was up to 8 weeks, and examined histologically. Ectopic cartilage was produced within the diffusion chamber after a total of 4 weeks but only if the first 2 weeks of recovery were in situ. With increasing time, calcified cartilage, osteoid, and bone were also observed. Overall, the evidence suggests that the cartilage forms via a direct conversion from tendon tissue and that the bone may form as a result of differentiative changes of hypertrophic chondrocytes.
将啮齿动物的跟腱进行中点切断术，在移除手术组织、放入微孔扩散室并腹腔内植入同基因宿主之前，让其在原位恢复不同时间。然后每周取出扩散室，使得手术后的总时间（即原位恢复时间加上在扩散室内的时间）最长达到8周，并进行组织学检查。总共4周后，扩散室内会产生异位软骨，但前提是最初2周的恢复是在原位进行的。随着时间的增加，还观察到了钙化软骨、类骨质和骨。总体而言，证据表明软骨是通过肌腱组织的直接转化形成的，而骨可能是肥大软骨细胞分化变化的结果。
