Kawada I, Takahashi K
Department of Internal Medicine, Kochi Municipal Central Hospital.
Nihon Rinsho. 1993 Mar;51(3):706-11.
Human eosinophils are known to exhibit autofluorescence, which allows the preparation of highly enriched eosinophil suspensions from peripheral blood of normal donors and bronchoaveolar lavage fluid, using flow cytometry (FCM). The sorted eosinophils were morphologically normal by light microscopy, the purity was more than 90% and the viability was more than 90%. The function of purified eosinophils was evaluated by chemotactic activity to platelet activating factor (PAF:10(-7) M) and recombinant human interleukin-5 (rhIL-5:10(3) ng/ml). Chemotactic activity was greater in purified eosinophils from patients with asthma than from normal subjects. Flow cytometric separation and analysis of eosinophils should thus, facilitate future studies of eosinophil heterogeneity and function.
已知人类嗜酸性粒细胞会表现出自发荧光，这使得利用流式细胞术（FCM）从正常供体的外周血和支气管肺泡灌洗液中制备高度富集的嗜酸性粒细胞悬液成为可能。通过光学显微镜观察，分选得到的嗜酸性粒细胞形态正常，纯度超过90%，活力超过90%。通过对血小板活化因子（PAF：10⁻⁷ M）和重组人白细胞介素-5（rhIL-5：10³ ng/ml）的趋化活性来评估纯化嗜酸性粒细胞的功能。哮喘患者纯化嗜酸性粒细胞的趋化活性高于正常受试者。因此，嗜酸性粒细胞的流式细胞术分离和分析应有助于未来对嗜酸性粒细胞异质性和功能的研究。
