Atallah R H, Kalman D A
Department of Environmental Health, University of Washington, Seattle 98195.
J Anal Toxicol. 1993 Mar-Apr;17(2):87-92. doi: 10.1093/jat/17.2.87.
A method has been developed for the determination of inorganic (InHg) and methylmercury (MeHg) in solubilized tissues with continuous-flow (flow injection) cold vapor atomic absorption spectrometry. Kidney, liver, and brain tissues were spiked with MeHg and InHg and solubilized at an elevated temperature in a solution containing 90 g/L NaOH, 2 g/L L-cysteine, and 4 g/L NaCl. Total mercury determination was achieved by continuous-flow cold vapor atomic absorption spectrometry using an inlet system containing a flow through photo-oxidation reactor and sodium borohydride as the mercury reductant. InHg was selectively determined in the presence of MeHg with this method when using stannous chloride as the reductant. MeHg concentrations were computed as the difference between the values obtained from the two analyses. Recoveries for spiked tissues were above 95% for InHg and MeHg. Quantitation limits for InHg and total mercury in tissues were 0.4 and 0.6 microgram/g, respectively. MeHg chloride levels from kidney tissues of exposed rats were evaluated using the present method in comparison with another method in which MeHg was measured using solvent extraction and capillary gas chromatography with electron capture detection.
已开发出一种用连续流动（流动注射）冷蒸气原子吸收光谱法测定溶解组织中无机汞（InHg）和甲基汞（MeHg）的方法。向肾脏、肝脏和脑组织中加入MeHg和InHg，并在含有90 g/L氢氧化钠、2 g/L L-半胱氨酸和4 g/L氯化钠的溶液中于高温下溶解。使用包含流通式光氧化反应器的进样系统和硼氢化钠作为汞还原剂，通过连续流动冷蒸气原子吸收光谱法进行总汞测定。当使用氯化亚锡作为还原剂时，用该方法可在MeHg存在下选择性测定InHg。MeHg浓度通过两次分析所得值的差值计算得出。加标组织中InHg和MeHg的回收率均高于95%。组织中InHg和总汞的定量限分别为0.4和0.6微克/克。使用本方法评估了暴露大鼠肾脏组织中的氯化甲基汞水平，并与另一种使用溶剂萃取和带电子捕获检测的毛细管气相色谱法测定MeHg的方法进行了比较。
