Briley P A, Eisenthal R, Harrison R, Smith G D
Biochem J. 1977 Feb 1;161(2):383-7. doi: 10.1042/bj1610383.
Diffluoro-oxaloacetate behaves as a competitive inhibitor of 2-oxoglutarate and as an uncompetitive inhibitor with respect to aspartate in steady-state kinetic experiments with cytoplasmic aspartate transaminase. In the presence of high concentrations of aspartate transaminase, difluoro-oxaloacetate is slowly transaminated to difluoro-aspartate, suggesting its use as a kinetic probe to study the reactions of the aminic form of the enzyme.
在对细胞质天冬氨酸转氨酶进行稳态动力学实验时，二氟草酰乙酸表现为2-氧代戊二酸的竞争性抑制剂，以及天冬氨酸的非竞争性抑制剂。在高浓度天冬氨酸转氨酶存在的情况下，二氟草酰乙酸会缓慢转氨生成二氟天冬氨酸，这表明它可作为一种动力学探针来研究该酶氨基形式的反应。
