二氟草酰乙酸与天冬氨酸转氨酶的相互作用。
Interaction of difluoro-oxaloacetate with aspartate transaminase.
作者信息
Briley P A, Eisenthal R, Harrison R, Smith G D
出版信息
Biochem J. 1977 Feb 1;161(2):383-7. doi: 10.1042/bj1610383.
Abstract
Diffluoro-oxaloacetate behaves as a competitive inhibitor of 2-oxoglutarate and as an uncompetitive inhibitor with respect to aspartate in steady-state kinetic experiments with cytoplasmic aspartate transaminase. In the presence of high concentrations of aspartate transaminase, difluoro-oxaloacetate is slowly transaminated to difluoro-aspartate, suggesting its use as a kinetic probe to study the reactions of the aminic form of the enzyme.
摘要
在对细胞质天冬氨酸转氨酶进行稳态动力学实验时，二氟草酰乙酸表现为2-氧代戊二酸的竞争性抑制剂，以及天冬氨酸的非竞争性抑制剂。在高浓度天冬氨酸转氨酶存在的情况下，二氟草酰乙酸会缓慢转氨生成二氟天冬氨酸，这表明它可作为一种动力学探针来研究该酶氨基形式的反应。
相似文献
1
Interaction of difluoro-oxaloacetate with aspartate transaminase.二氟草酰乙酸与天冬氨酸转氨酶的相互作用。
Biochem J. 1977 Feb 1;161(2):383-7. doi: 10.1042/bj1610383.
2
Reaction of the aminic form of aspartate transaminase with difluoro-oxaloacetate.天冬氨酸转氨酶氨基形式与二氟草酰乙酸的反应。
Biochem J. 1977 Oct 1;167(1):193-200. doi: 10.1042/bj1670193.
3
[19F]fluorine nuclear-magnetic-resonance study of the interaction of difluoro-oxaloacetate with aspartate transaminase.[19F]氟核磁共振研究二氟草酰乙酸与天冬氨酸转氨酶的相互作用
Biochem J. 1977 May 1;163(2):325-31. doi: 10.1042/bj1630325.
4
[19F]Fluorine nuclear-magnetic-resonance study of the reaction of the aminic form of aspartate transaminase with diffuro-oxaloacetate.[19F]氟核磁共振研究天冬氨酸转氨酶氨基形式与二氟草酰乙酸的反应。
Biochem Soc Trans. 1978;6(1):190-3. doi: 10.1042/bst0060190.
5
Immobilized glutamate oxaloacetate transaminase. Steady state kinetic analysis and stability studies.固定化谷氨酸草酰乙酸转氨酶。稳态动力学分析与稳定性研究。
Biochim Biophys Acta. 1975 Sep 22;403(1):79-88. doi: 10.1016/0005-2744(75)90010-8.
6
Kinetic mechanism of Escherichia coli isocitrate dehydrogenase and its inhibition by glyoxylate and oxaloacetate.大肠杆菌异柠檬酸脱氢酶的动力学机制及其受乙醛酸和草酰乙酸的抑制作用
Biochem J. 1986 Mar 1;234(2):317-23. doi: 10.1042/bj2340317.
7
Effects of oxaloacetate and L-glutamate on glyceraldehyde 3-phosphate inhibition of aspartate aminotransferase isozymes as measured by a 2oxoglutarate dehydrogenase coupled assay.通过2-氧代戊二酸脱氢酶偶联测定法测量草酰乙酸和L-谷氨酸对3-磷酸甘油醛抑制天冬氨酸氨基转移酶同工酶的影响。
Eur J Biochem. 1971 Nov 11;23(2):314-20. doi: 10.1111/j.1432-1033.1971.tb01623.x.
8
The anomalous kinetics of coupled aspartate aminotransferase and malate dehydrogenase. Evidence for compartmentation of oxaloacetate.天冬氨酸转氨酶与苹果酸脱氢酶偶联反应的异常动力学。草酰乙酸区室化的证据。
Biochem J. 1976 Mar 1;153(3):571-7. doi: 10.1042/bj1530571.
9
GLUTAMIC-ASPARTIC TRANSAMINASE. 8. EQUILIBRIUM KINETICS WITH ASPARTATE.谷氨酸-天冬氨酸转氨酶。8. 与天冬氨酸的平衡动力学
J Biol Chem. 1965 Jul;240:2907-13.
10
[ON THE CHANGE OF THE GLUTAMATE-OXALOACETATE TRANSAMINASE ACTIVITY AND THE GLUTAMATE-PYRUVATE TRANSAMINASE ACTIVITY BY THE TYRAMINE INJECTION].
Seishin Igaku Kenkyusho Gyosekishu. 1964;11:43-50.
引用本文的文献
1
Determination of half-reaction equilibrium in a ping-pong enzyme mechanism.乒乓酶机制中半反应平衡的测定
Neurochem Res. 1996 Sep;21(9):1061-4. doi: 10.1007/BF02532416.
2
Reaction of the aminic form of aspartate transaminase with difluoro-oxaloacetate.天冬氨酸转氨酶氨基形式与二氟草酰乙酸的反应。
Biochem J. 1977 Oct 1;167(1):193-200. doi: 10.1042/bj1670193.
3
[19F]fluorine nuclear-magnetic-resonance study of the interaction of difluoro-oxaloacetate with aspartate transaminase.[19F]氟核磁共振研究二氟草酰乙酸与天冬氨酸转氨酶的相互作用
Biochem J. 1977 May 1;163(2):325-31. doi: 10.1042/bj1630325.
本文引用的文献
1
Interaction between beta-fluoro-oxaloacetate and glutamate-aspartate transaminase of heart mitochondria.
J Biol Chem. 1960 Feb;235:416-9.
2
Glutamic aspartic transaminase. I. Assay, purification, and general properties.谷氨酸天冬氨酸转氨酶。I. 测定、纯化及一般性质。
J Biol Chem. 1959 Jan;234(1):51-7.
3
The complete amino acid sequence of cytoplasmic aspartate aminotransferase from pig heart.猪心脏胞质天冬氨酸转氨酶的完整氨基酸序列。
FEBS Lett. 1973 Jan 1;29(1):31-34. doi: 10.1016/0014-5793(73)80008-0.
4
On the formation of S-(alpha,beta-dicarboxyethyl) derivatives of glutamic-aspartic aminotransferase.关于谷氨酸 - 天冬氨酸转氨酶的S -（α，β - 二羧乙基）衍生物的形成
Biochem Biophys Res Commun. 1964 Jun 15;16(3):221-6. doi: 10.1016/0006-291x(64)90329-8.
5
Use of multivariate non-linear regression analysis in fitting enzyme kinetic models. An empirical study of the inhibition of aspartate aminotransferase by dicarboxylic acid substrate analogues.多元非线性回归分析在酶动力学模型拟合中的应用。二羧酸底物类似物对天冬氨酸转氨酶抑制作用的实证研究。
J Theor Biol. 1969 Jan;22(1):117-50. doi: 10.1016/0022-5193(69)90083-6.
6
The molecular weight and other properties of aspartate aminotransferase from pig heart muscle.猪心肌中天冬氨酸转氨酶的分子量及其他性质。
Eur J Biochem. 1968 Sep 24;5(4):528-39. doi: 10.1111/j.1432-1033.1968.tb00402.x.
7
Structure and catalytic role of the functional groups of aspartate aminotransferase.天冬氨酸转氨酶功能基团的结构与催化作用
Vitam Horm. 1970;28:157-94. doi: 10.1016/s0083-6729(08)60893-6.
8
The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.直接线性作图法。一种用于估算酶动力学参数的新的图形方法。
Biochem J. 1974 Jun;139(3):715-20. doi: 10.1042/bj1390715.
Zotero 插件