Smith G D, Harrison R, Eisenthal R
Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, United Kingdom.
Neurochem Res. 1996 Sep;21(9):1061-4. doi: 10.1007/BF02532416.
Substituted enzyme (or ping-pong) mechanisms usually involve enzymes that exist in two forms that alternate during the catalytic reaction. A method is described here for determining the position of the equilibrium of a half reaction in a ping-pong enzyme mechanism that is based on the kinetics of the burst reaction which occurs upon addition of reactants that recycle the enzyme from one form to another. The theoretical basis for the analysis is developed, and the method is applied to the half reaction of the aldimine form of aspartate transaminase with difluoro-oxaloacetate.
取代酶（或乒乓）机制通常涉及在催化反应过程中以两种交替形式存在的酶。本文描述了一种基于突发反应动力学来确定乒乓酶机制中半反应平衡位置的方法，该突发反应发生在添加使酶从一种形式循环到另一种形式的反应物时。阐述了该分析方法的理论基础，并将其应用于天冬氨酸转氨酶醛亚胺形式与二氟草酰乙酸的半反应。
