昆虫飞行肌Z线中的蛋白质。
The proteins in the Z line of insect flight muscle.
作者信息
Bullard B, Sainsbury G M
出版信息
Biochem J. 1977 Feb 1;161(2):399-403. doi: 10.1042/bj1610399.
Abstract
Z discs were isolated from Lethocerus flight muscle by removing the contractile proteins from myofibrils with a solution of high ionic strength. The protein composition of the Z discs was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the major proteins were alpha-actinin, actin and tropomyosin. Z lines were selectively removed from intact myofibrils by digestion with crude lipase and chymotrypsin, but not by purified lipase.
摘要
通过用高离子强度溶液从肌原纤维中去除收缩蛋白，从田鳖飞行肌中分离出Z盘。用十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析Z盘的蛋白质组成；主要蛋白质是α-辅肌动蛋白、肌动蛋白和原肌球蛋白。通过用粗脂肪酶和胰凝乳蛋白酶消化，可从完整的肌原纤维中选择性去除Z线，但用纯化的脂肪酶则不能。
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