兔肝“L”型丙酮酸激酶的作用机制。反对磷酸酶形成的证据。
Mechanism of "L"-type pyruvate kinase from rabbit liver. Evidence against phosphoenzyme formation.
作者信息
Dann L G, Britton H G
出版信息
Biochem J. 1977 Feb 1;161(2):445-8. doi: 10.1042/bj1610445.
Abstract
The "L"-type pyruvate kinase from rabbit liver does not catalyse exchange between phosphoenol[1-14C]pyruvate and pyruvate at either pH 8.5 or 6.2. Spectrophotometric experiments at pH 8.5 and 6;2 and gel-filtration experiments with [32P]phosphoenolpyruvate at pH 8,5 also fail to demonstrate phosphoenzyme formation. It is concluded that it is very unlikely that the enzyme has a phosphoenzyme mechanism.
摘要
来自兔肝的“L”型丙酮酸激酶在pH 8.5或6.2时均不催化磷酸烯醇式[1-¹⁴C]丙酮酸与丙酮酸之间的交换。在pH 8.5和6.2进行的分光光度实验以及在pH 8.5用[³²P]磷酸烯醇式丙酮酸进行的凝胶过滤实验也未能证明磷酸酶的形成。得出的结论是，该酶极不可能具有磷酸酶机制。
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