Grzybowski J, Will D W, Randall R E, Smith C A, Brown T
Edinburgh Centre for Molecular Recognition, Department of Chemistry, University of Edinburgh, UK.
Nucleic Acids Res. 1993 Apr 25;21(8):1705-12. doi: 10.1093/nar/21.8.1705.
A series of non-nucleoside-based 2,4-dinitrophenyl (DNP) phosphoramidites have been prepared and used in the multiple labelling of oligonucleotides during solid-phase synthesis. The length of spacer arm between the DNP label and the oligonucleotide phosphate backbone, and the number of attached DNP groups have both been varied in order to determine the optimum conditions for anti-DNP antibody binding. Detection using enzyme-linked colorimetric techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.
已制备了一系列基于非核苷的2,4-二硝基苯基（DNP）亚磷酰胺，并将其用于固相合成过程中寡核苷酸的多重标记。为了确定抗DNP抗体结合的最佳条件，DNP标记与寡核苷酸磷酸主链之间的间隔臂长度以及连接的DNP基团数量均已改变。使用酶联比色技术进行检测显示，其灵敏度与使用生物素化寡核苷酸所能获得的灵敏度相当。
