McClean J, Davison A, Rao M V, Brown T
Department of Chemistry, University of Southampton, Highfield, England, U.K.
Biomed Pept Proteins Nucleic Acids. 1996;2(1):7-12.
DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.
二硝基苯酚（DNP）标记的亚磷酰胺已被用于合成带有多个DNP报告基团的寡核苷酸。人们对这些标记寡核苷酸形成的双链体的抗体介导检测及其稳定性进行了研究。一种DNP标记的脱氧尿苷三磷酸也已被用于通过聚合酶链反应将DNP标记酶促掺入DNA中。人们还研究了在聚合酶链反应中使用DNP标记引物的情况。
