含3-脱氮腺嘌呤：胸腺嘧啶碱基对的寡脱氧核糖核苷酸双链体的热力学稳定性和药物结合特性。
Thermodynamic stability and drug-binding properties of oligodeoxyribonucleotide duplexes containing 3-deazaadenine:thymine base pairs.
作者信息
Lever C, Li X, Cosstick R, Ebel S, Brown T
机构信息
Robert Robinson Laboratories, Department of Chemistry, University of Liverpool, UK.
出版信息
Nucleic Acids Res. 1993 Apr 25;21(8):1743-6. doi: 10.1093/nar/21.8.1743.
Abstract
We have used ultraviolet melting techniques to study the effect on stability of incorporating the nucleoside analogue 2'-deoxy-3-deazaadenosine (d3cA) into the duplex 5'-d(CGCAATCG)-3'-d(GCGTTAGC). Our results demonstrate that the successive replacement of dA by d3CA increasingly destabilises the duplex. The destabilising effect of this analogue is considerably enhanced as the pH is lowered and the results are consistent with protonation of 3-deazaadenine (probably at N-1) contributing to duplex destablisation. Surprisingly, the incorporation of d3CA does not significantly affect the binding of distamycin-A.
摘要
我们利用紫外熔解技术研究了将核苷类似物2'-脱氧-3-脱氮腺苷（d3cA）掺入双链体5'-d（CGCAATCG）-3'-d（GCGTTAGC）中对其稳定性的影响。我们的结果表明，用d3CA连续取代dA会使双链体越来越不稳定。随着pH值降低，这种类似物的去稳定作用显著增强，结果与3-脱氮腺嘌呤（可能在N-1位）的质子化导致双链体去稳定一致。令人惊讶的是，d3CA的掺入对双氢链霉素-A的结合没有显著影响。
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