涉及2,6-二氨基嘌呤碱基配对的热力学研究。
Thermodynamic studies of base pairing involving 2,6-diaminopurine.
作者信息
Cheong C, Tinoco I, Chollet A
机构信息
Department of Chemistry, University of California, Berkeley 94720.
出版信息
Nucleic Acids Res. 1988 Jun 10;16(11):5115-22. doi: 10.1093/nar/16.11.5115.
Abstract
The thermal stabilities of oligodeoxyribonucleotide duplexes containing 2,6-diaminopurine (D) matched with each of the four normal DNA bases were determined by optical melting techniques. Comparison of optical melting curves yielded relative stabilities for the D-containing standard base pairs in an otherwise identical base-pair sequence. The D:T pair was found to be more stable than the A:T pair in dC3DG3:dC3TG3, as stable as the A:T in dCT3DT3G:dCA3TA3G, and less stable than the A:T in dCA3DA3G:dCT7G. The order of stabilities for X:Y in the DNA duplex dCA3XA3G:dCT3YT3G is: (A:T) greater than (T:D) congruent to (D:T) greater than or equal to (T:A) greater than (C:D) congruent to (D:A) congruent to (D:G) greater than or equal to (D:C) congruent to (G:D) congruent to (D:D) greater than or equal to (A:D). Implications of these results for design of DNA oligonucleotide probes are discussed.
摘要
通过光学熔解技术测定了含有与四种正常DNA碱基配对的2,6 - 二氨基嘌呤（D）的寡脱氧核糖核苷酸双链体的热稳定性。通过比较光学熔解曲线，得出了在其他方面相同的碱基对序列中含D的标准碱基对的相对稳定性。发现在dC3DG3:dC3TG3中，D:T对比A:T对更稳定；在dCT3DT3G:dCA3TA3G中，D:T对与A:T对一样稳定；在dCA3DA3G:dCT7G中，D:T对比A:T对不稳定。在DNA双链体dCA3XA3G:dCT3YT3G中，X:Y的稳定性顺序为：（A:T）大于（T:D）等同于（D:T）大于或等于（T:A）大于（C:D）等同于（D:A）等同于（D:G）大于或等于（D:C）等同于（G:D）等同于（D:D）大于或等于（A:D）。讨论了这些结果对DNA寡核苷酸探针设计的意义。
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