Cheong C, Tinoco I, Chollet A
Department of Chemistry, University of California, Berkeley 94720.
Nucleic Acids Res. 1988 Jun 10;16(11):5115-22. doi: 10.1093/nar/16.11.5115.
The thermal stabilities of oligodeoxyribonucleotide duplexes containing 2,6-diaminopurine (D) matched with each of the four normal DNA bases were determined by optical melting techniques. Comparison of optical melting curves yielded relative stabilities for the D-containing standard base pairs in an otherwise identical base-pair sequence. The D:T pair was found to be more stable than the A:T pair in dC3DG3:dC3TG3, as stable as the A:T in dCT3DT3G:dCA3TA3G, and less stable than the A:T in dCA3DA3G:dCT7G. The order of stabilities for X:Y in the DNA duplex dCA3XA3G:dCT3YT3G is: (A:T) greater than (T:D) congruent to (D:T) greater than or equal to (T:A) greater than (C:D) congruent to (D:A) congruent to (D:G) greater than or equal to (D:C) congruent to (G:D) congruent to (D:D) greater than or equal to (A:D). Implications of these results for design of DNA oligonucleotide probes are discussed.
通过光学熔解技术测定了含有与四种正常DNA碱基配对的2,6 - 二氨基嘌呤(D)的寡脱氧核糖核苷酸双链体的热稳定性。通过比较光学熔解曲线,得出了在其他方面相同的碱基对序列中含D的标准碱基对的相对稳定性。发现在dC3DG3:dC3TG3中,D:T对比A:T对更稳定;在dCT3DT3G:dCA3TA3G中,D:T对与A:T对一样稳定;在dCA3DA3G:dCT7G中,D:T对比A:T对不稳定。在DNA双链体dCA3XA3G:dCT3YT3G中,X:Y的稳定性顺序为:(A:T)大于(T:D)等同于(D:T)大于或等于(T:A)大于(C:D)等同于(D:A)等同于(D:G)大于或等于(D:C)等同于(G:D)等同于(D:D)大于或等于(A:D)。讨论了这些结果对DNA寡核苷酸探针设计的意义。