De Sole P, Fresu R, Frigieri L, Pagliari G, De Simone C, Guerriero C
Clinical Chemistry, Policlinico Gemelli, Università Cattolica del Sacro Cuore, Roma, Italy.
J Biolumin Chemilumin. 1993 May-Jun;8(3):153-8. doi: 10.1002/bio.1170080305.
The chemiluminescence of peripheral blood monocytes and alveolar macrophages was determined in the presence of luminol and lucigenin, either before or after the cell adherence to the luminometer curvettes. In the case of monocytes, cell adherence induces an increase of luminol-dependent chemiluminescence and has almost no effect on the lucigenin-dependent chemiluminescence. However, it shows a strong inhibition of the lucigenin-dependent chemiluminescence and almost no effect on luminol-dependent chemiluminescence, in the case of alveolar macrophages. These results show that adhesion to plastic alters the metabolic burst of both monocytes and alveolar macrophages. Although the mechanisms are poorly understood, they seem to be related to the modifications that take place during the differentiation of peripheral monocytes to alveolar macrophages.
在鲁米诺和光泽精存在的情况下，于细胞粘附到发光计比色皿之前或之后，测定外周血单核细胞和肺泡巨噬细胞的化学发光。对于单核细胞而言，细胞粘附会导致鲁米诺依赖性化学发光增加，而对光泽精依赖性化学发光几乎没有影响。然而，对于肺泡巨噬细胞，它显示出对光泽精依赖性化学发光有强烈抑制作用，而对鲁米诺依赖性化学发光几乎没有影响。这些结果表明，与塑料的粘附会改变单核细胞和肺泡巨噬细胞的代谢爆发。尽管其机制尚不清楚，但它们似乎与外周单核细胞分化为肺泡巨噬细胞过程中发生的变化有关。
