Aasen T B, Bolann B, Glette J, Ulvik R J, Schreiner A
Department of Thoracic Medicine, Haukeland Hospital, University of Bergen, Norway.
Scand J Clin Lab Invest. 1987 Nov;47(7):673-9.
A method for the measurement of reactive oxygen species generated by activated mononuclear phagocytes by use of lucigenin-dependent chemiluminescence was developed. Opsonized zymosan was used as a stimulant to evaluate the chemiluminescence response of monocytes. A cell-free system in which superoxide anion and hydrogen peroxide were produced by the xanthine-xanthine oxidase reaction was used to examine the role of these radical species in the excitation of lucigenin, and to standardize the chemiluminescence method. It was found that the light emission in lucigenin-dependent chemiluminescence was evoked by the superoxide anion, but other radicals may also be active.
开发了一种利用基于光泽精的化学发光来测量活化单核吞噬细胞产生的活性氧的方法。用调理酵母聚糖作为刺激物来评估单核细胞的化学发光反应。使用通过黄嘌呤-黄嘌呤氧化酶反应产生超氧阴离子和过氧化氢的无细胞系统，以研究这些自由基在光泽精激发中的作用，并对化学发光方法进行标准化。结果发现，基于光泽精的化学发光中的发光是由超氧阴离子引起的，但其他自由基也可能具有活性。
