van Vlijmen-Willems I, van Erp P
Department of Dermatology, University Hospital Nijmegen, The Netherlands.
Biotech Histochem. 1993 Mar;68(2):67-74. doi: 10.3109/10520299309104669.
We describe a method in which microwave irradiation is used to reduce substantially the incubation time for immunoperoxidase staining of antigens in cryostat sections of psoriatic skin. An incubation time of 5-9 min irradiation at 80 W generated similar or better staining intensity for all antibodies used compared to the standard methods using 30-60 min incubation at room temperature. Although we found that microwave irradiation could be used with all antibodies tested, independent of whether they recognized extracellular, membrane or cytoplasmic antigens in skin, the conditions needed to be optimized for each antibody.
我们描述了一种方法，其中利用微波辐射显著缩短了对银屑病皮肤冰冻切片中抗原进行免疫过氧化物酶染色的孵育时间。与在室温下孵育30 - 60分钟的标准方法相比，在80瓦功率下照射5 - 9分钟的孵育时间，对所有使用的抗体产生了相似或更好的染色强度。尽管我们发现微波辐射可用于所有测试的抗体，无论它们识别的是皮肤中的细胞外、膜或细胞质抗原，但每种抗体所需的条件都需要优化。
