Yamada O, Oshimi K, Mizoguchi H
Department of Medicine, Tokyo Women's Medical College, Japan.
Int J Hematol. 1993 Apr;57(2):181-6.
The broken ends of chromosomes are unstable, and tandem fusion of telomeres has been observed in some tumors. Using Southern blot analysis, we report here telomeric DNA changes in hematologic cells. There was some variation in the length of the telomeric DNA in normal peripheral blood mononuclear cells obtained from four different individuals, ranging from 10 to 12 kilobases (kb), but there was little difference in signal strength. In two cell lines tested, HL-60 and K562, there was a telomeric sequence reduction of 2-4 kb and there was also a diminution of signal intensity. Reduction of the telomeric DNA array was also observed in two leukemic cases tested. The peak telomere length of the leukemic cells was 5 and 4 kb before and 10 and 7 kb after treatment, respectively, and in one case there was also a reduction in copy numbers of about 50%. Since no remarkable changes were detected in the Alu and alphoid sequences in either normal or leukemic cells, it appeared that the DNA change was specific to telomeric regions. Assessment of telomeric DNA changes may aid in determining the biological significance of leukemic cells.
染色体的断裂末端不稳定，并且在一些肿瘤中观察到了端粒的串联融合。通过Southern印迹分析，我们在此报告血液学细胞中端粒DNA的变化。从四个不同个体获得的正常外周血单核细胞中端粒DNA的长度存在一些差异，范围为10至12千碱基（kb），但信号强度差异不大。在测试的两种细胞系HL-60和K562中，端粒序列减少了2 - 4 kb，信号强度也有所减弱。在测试的两例白血病病例中也观察到端粒DNA阵列的减少。白血病细胞的端粒长度峰值在治疗前分别为5 kb和4 kb，治疗后分别为10 kb和7 kb，并且在一例中拷贝数也减少了约50%。由于在正常细胞或白血病细胞的Alu和α卫星序列中均未检测到明显变化，因此似乎DNA变化是端粒区域特有的。端粒DNA变化的评估可能有助于确定白血病细胞的生物学意义。
