Takauchi K, Tashiro S, Ohtaki M, Kamada N
Department of Hematology, Hiroshima University.
Jpn J Cancer Res. 1994 Feb;85(2):127-30. doi: 10.1111/j.1349-7006.1994.tb02071.x.
The length of telomere restriction fragments (TRF) was studied by Southern blotting in 42 patients with acute myelocytic leukemia (AML) including 15 patients with 8;21 translocation, 8 with 15;17 translocation and 19 with a normal karyotype. The TRF length of leukemic cells with a normal karyotype and with 8;21 or 15;17 translocation showed significant reduction compared to that of lymphocytes from normal individuals (P < 0.05). In addition, there was a statistically significant difference in TRF length between leukemic cells with a normal karyotype and 8;21 translocation (P < 0.05). Therefore, the significant difference of telomere reduction in 8;21 translocation may be an important factor in the leukemogenic process.
通过Southern印迹法研究了42例急性髓细胞白血病（AML）患者的端粒限制片段（TRF）长度，其中包括15例有8;21易位的患者、8例有15;17易位的患者和19例核型正常的患者。与正常个体的淋巴细胞相比，核型正常以及有8;21或15;17易位的白血病细胞的TRF长度显著缩短（P < 0.05）。此外，核型正常的白血病细胞与有8;21易位的白血病细胞之间的TRF长度存在统计学显著差异（P < 0.05）。因此，8;21易位中端粒缩短的显著差异可能是白血病发生过程中的一个重要因素。
