Roca A L, Weaver D R, Reppert S M
Laboratory of Developmental Chronobiology, Children's Service, Massachusetts General Hospital, Boston 02114.
Brain Res. 1993 Apr 9;608(1):159-65. doi: 10.1016/0006-8993(93)90789-p.
Serotonin (5HT) is thought to reset the biological clock in the suprachiasmatic nuclei (SCN) in vitro through a postsynaptic 5HT-1a receptor. Thus we examined 5HT receptor gene expression in the SCN by in situ hybridization. On film autoradiograms, 5HT-1c receptor mRNA showed intense SCN hybridization, while 5HT-1b receptor mRNA displayed a weaker signal. Emulsion autoradiograms additionally revealed expression of 5HT-1a and 5HT-2 receptor mRNAs by a few scattered SCN cells. 5HT-3 receptor mRNA was not detected in the SCN, although the transcript was detected elsewhere in the brain. 5HT-1d and -1e receptor mRNAs were not detected in the SCN or elsewhere in brain within the sections examined. The results do not support a major role for postsynaptic 5HT-1a receptors in resetting SCN rhythms.
血清素（5HT）被认为在体外通过突触后5HT-1a受体重置视交叉上核（SCN）中的生物钟。因此，我们通过原位杂交检测了SCN中5HT受体基因的表达。在胶片放射自显影片上，5HT-1c受体mRNA显示出强烈的SCN杂交信号，而5HT-1b受体mRNA显示出较弱的信号。乳胶放射自显影片还显示，少数散在的SCN细胞表达5HT-1a和5HT-2受体mRNA。尽管在脑内其他部位检测到了5HT-3受体mRNA的转录本，但在SCN中未检测到。在所检查的切片中，未在SCN或脑内其他部位检测到5HT-1d和-1e受体mRNA。这些结果不支持突触后5HT-1a受体在重置SCN节律中起主要作用。
