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Methylation of DNA in rat liver and intestine by dimethylnitrosamine and N-methylnitrosourea.

作者信息

Daoud A H, Irving C C

出版信息

Chem Biol Interact. 1977 Feb;16(2):135-43. doi: 10.1016/0009-2797(77)90123-5.

DOI:10.1016/0009-2797(77)90123-5
PMID:849620
Abstract

A chromatographic procedure for improved separation of deoxyribonucleosides and methylated deoxyribonucleosides is described. DNA was isolated from liver and small intestine of rats treated with [14C]dimethylnitrosamine ([14C]DMN) or N-[3H]methyl-N-nitrosourea ([3H]MNU), and the purified DNA was hydrolyzed enzymatically. The deoxyribonucleosides were chromatographed on an Aminex A-6 cation exchange column at 37 degrees C with 0.4 M ammonium formate, pH 4.5, as eluant. In addition to showing the presence of the expected alkylated products, N7-methyldeoxyguanosine (determined as N7-methylguanine) and O6-methyldeoxyguanosine, several other minor methylated products were found in liver and intestinal DNA of rats treated with DMN or MNU. Two of these products are believed to be N3-methylthymidine and O4-methylthymidine.

摘要

相似文献

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