Lantz R J, Farid K Z, Koons J, Tenbarge J B, Bopp R J
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285.
J Chromatogr. 1993 Apr 21;614(1):175-9. doi: 10.1016/0378-4347(93)80239-z.
A capillary gas chromatographic method with 63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid-liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sensitivity of detection. A 30 m x 0.25 mm I.D. DB-17 capillary column resolves the compounds from endogenous matrix interferences. The limit of quantitation by this method is 5 ng/ml for each compound. Stability studies show that fluoxetine and norfluoxetine are stable in human plasma for up to 96 h at room temperature and up to one year at -20 degrees C.
