Tsikas D, Velásquez R, Toledano C, Brunner G
Department of Gastroenterology and Hepatology, Hannover Medical School, Germany.
J Chromatogr. 1993 Apr 21;614(1):37-41. doi: 10.1016/0378-4347(93)80221-o.
A procedure for the selective ion-pair extraction of histamine from various biological fluids and tissues by using the ion-pairing agent bis(2-ethylhexyl)hydrogen-phosphoric acid has been developed. This procedure enabled routine determination of histamine in plasma, urine and tissue samples of humans and rats by reversed-phase high-performance liquid chromatography with fluorescence detection using o-phthaldialdehyde.
已开发出一种使用离子对试剂双(2-乙基己基)磷酸从各种生物流体和组织中选择性离子对萃取组胺的方法。该方法能够通过使用邻苯二甲醛的反相高效液相色谱荧光检测法对人和大鼠的血浆、尿液及组织样本中的组胺进行常规测定。