Holladay F P, Wood G W
Department of Surgery, University of Kansas Medical Center, Kansas City 66160-7410.
J Neuroimmunol. 1993 Apr;44(1):27-32. doi: 10.1016/0165-5728(93)90264-y.
The study demonstrated that RT2, a highly malignant anaplastic glioma, expresses antigens that make it susceptible to in vivo adoptive immunotherapy with cytotoxic T lymphocyte-containing immune cell populations. Rats were immunized with irradiated RT2 tumor cells and the adjuvant C. parvum. Lymphocytes from immunized rats were restimulated in vitro with irradiated RT2 tumor cells plus interleukin-2 (IL-2). The cells that proliferated and differentiated in vitro effectively killed RT2, but only low levels of cytotoxicity were observed against other histopathologically related and unrelated, syngeneic and allogeneic target cells. Adoptive transfer of immune cells combined with a 5-day course of systemic IL-2 produced specific regression of brain tumors growing as lung microfoci.
该研究表明，RT2作为一种高度恶性的间变性胶质瘤，表达的抗原使其易受含细胞毒性T淋巴细胞的免疫细胞群体进行的体内过继性免疫治疗的影响。用经辐照的RT2肿瘤细胞和佐剂微小隐孢子虫对大鼠进行免疫。用经辐照的RT2肿瘤细胞加白细胞介素-2（IL-2）在体外对免疫大鼠的淋巴细胞进行再刺激。在体外增殖和分化的细胞有效杀伤了RT2，但对其他组织病理学相关和不相关的同基因和异基因靶细胞仅观察到低水平的细胞毒性。免疫细胞的过继性转移与为期5天的全身性IL-2疗程相结合，使作为肺微病灶生长的脑肿瘤发生特异性消退。
