Grenier D, Groleau D, Nanci A
Département de Santé Buccale, Faculté de Médecine Dentaire, Université de Montréal, Québec, Canada.
J Periodontal Res. 1993 May;28(3):211-8. doi: 10.1111/j.1600-0765.1993.tb01071.x.
Lectins were used to characterize glycoconjugates on the cell surface of Treponema denticola, a suspected periodontopathogen. Bacteria were first screened by light microscopy using fluorescein isothiocyanate-coupled lectins. Wheat germ agglutinin (WGA) showed a high reactivity to T. denticola. While the WGA-binding activity was accentuated following heating or detergent treatments of bacterial cells, the reaction was inhibited by incorporation of competing carbohydrates. Scanning and transmission electron microscope studies were conducted in order to characterize the distribution of the WGA-binding sites on the cell surface of T. denticola. Data from these studies confirmed that heat treatment increases the percentage of labeled profiles and suggest that the WGA-binding sites are concentrated on specific regions on the spirochete surface. Initial biochemical analysis indicated that the high reactivity to WGA resides in a peptidoglycan fraction.
凝集素被用于表征可疑的牙周病原体——齿垢密螺旋体细胞表面的糖结合物。首先使用异硫氰酸荧光素偶联的凝集素通过光学显微镜对细菌进行筛选。麦胚凝集素（WGA）对齿垢密螺旋体显示出高反应性。虽然在对细菌细胞进行加热或去污剂处理后WGA结合活性增强，但该反应可被加入竞争性碳水化合物所抑制。为了表征WGA结合位点在齿垢密螺旋体细胞表面的分布，进行了扫描电子显微镜和透射电子显微镜研究。这些研究的数据证实热处理增加了标记轮廓的百分比，并表明WGA结合位点集中在螺旋体表面的特定区域。初步生化分析表明，对WGA的高反应性存在于肽聚糖部分。
