1型人类免疫缺陷病毒gp160和gp120包膜糖蛋白的二级结构：傅里叶变换红外光谱研究
Secondary structure of gp160 and gp120 envelope glycoproteins of human immunodeficiency virus type 1: a Fourier transform infrared spectroscopic study.
作者信息
Decroly E, Cornet B, Martin I, Ruysschaert J M, Vandenbranden M
机构信息
Laboratoire de Chimie-Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Belgium.
出版信息
J Virol. 1993 Jun;67(6):3552-60. doi: 10.1128/JVI.67.6.3552-3560.1993.
Abstract
The secondary structure of the precursor (gp160) of the envelope protein of human immunodeficiency virus type 1 (BH10) and its receptor-binding subunit (gp120) was studied by Fourier-transformed attenuated total reflection spectroscopy. A higher alpha-helix/beta-sheet ratio in the gp120 subunit than in the precursor indicates a structural heterogeneity between the two subunits (gp120 and gp41), in agreement with classical secondary-structure predictions. The secondary structure of gp41 was estimated and compared with existing models. The high alpha-helical content in gp41 and the dominant beta-sheet content in gp120 resemble the distribution in influenza virus hemagglutinin subunits.
摘要
利用傅里叶变换衰减全反射光谱法研究了1型人类免疫缺陷病毒（BH10）包膜蛋白前体（gp160）及其受体结合亚基（gp120）的二级结构。与经典二级结构预测结果一致，gp120亚基中的α-螺旋/β-折叠比例高于前体，这表明两个亚基（gp120和gp41）之间存在结构异质性。对gp41的二级结构进行了估算，并与现有模型进行了比较。gp41中高含量的α-螺旋和gp120中占主导地位的β-折叠含量类似于流感病毒血凝素亚基中的分布。
相似文献
1
Secondary structure of gp160 and gp120 envelope glycoproteins of human immunodeficiency virus type 1: a Fourier transform infrared spectroscopic study.1型人类免疫缺陷病毒gp160和gp120包膜糖蛋白的二级结构：傅里叶变换红外光谱研究
J Virol. 1993 Jun;67(6):3552-60. doi: 10.1128/JVI.67.6.3552-3560.1993.
2
Determinants of human immunodeficiency virus type 1 envelope glycoprotein oligomeric structure.人类免疫缺陷病毒1型包膜糖蛋白寡聚体结构的决定因素。
J Virol. 1995 Feb;69(2):1209-18. doi: 10.1128/JVI.69.2.1209-1218.1995.
3
Computer analysis of human immunodeficiency virus-1 envelope glycoprotein: functional topogenic domains as signals for transfer and cleavage.人类免疫缺陷病毒1型包膜糖蛋白的计算机分析：作为转运和切割信号的功能性拓扑结构域
Virus Genes. 1991 Oct;5(4):287-312. doi: 10.1007/BF00271529.
4
Biological properties of recombinant HIV envelope synthesized in CHO glycosylation-mutant cell lines.在CHO糖基化突变细胞系中合成的重组HIV包膜的生物学特性。
Virology. 1996 Apr 1;218(1):224-31. doi: 10.1006/viro.1996.0182.
5
Analysis of endoproteolytic cleavage and intracellular transport of human immunodeficiency virus type 1 envelope glycoproteins using mutant CD4 molecules bearing the transmembrane endoplasmic reticulum retention signal.利用携带跨膜内质网滞留信号的突变型CD4分子分析1型人类免疫缺陷病毒包膜糖蛋白的内切蛋白水解切割及细胞内运输
J Gen Virol. 1993 Oct;74 ( Pt 10):2085-97. doi: 10.1099/0022-1317-74-10-2085.
6
Sulfation of the human immunodeficiency virus envelope glycoprotein.人类免疫缺陷病毒包膜糖蛋白的硫酸化作用。
J Virol. 1992 Dec;66(12):6953-9. doi: 10.1128/JVI.66.12.6953-6959.1992.
7
Complex-type N-linked oligosaccharides of gp120 from human immunodeficiency virus type 1 contain sulfated N-acetylglucosamine.来自1型人类免疫缺陷病毒的gp120的复合型N-连接寡糖含有硫酸化的N-乙酰葡糖胺。
J Virol. 1993 Feb;67(2):943-52. doi: 10.1128/JVI.67.2.943-952.1993.
8
gp160, the envelope glycoprotein of human immunodeficiency virus type 1, is a dimer of 125-kilodalton subunits stabilized through interactions between their gp41 domains.gp160是1型人类免疫缺陷病毒的包膜糖蛋白，它是由125千道尔顿亚基组成的二聚体，通过其gp41结构域之间的相互作用而稳定。
J Virol. 1991 Jul;65(7):3797-803. doi: 10.1128/JVI.65.7.3797-3803.1991.
9
10
The envelope of HIV-1 as a key component to infectivity.HIV-1包膜作为感染性的关键组成部分。
Prog AIDS Pathol. 1990;2:1-11.
引用本文的文献
1
Characterization of the fusion core in zebrafish endogenous retroviral envelope protein.斑马鱼内源性逆转录病毒包膜蛋白中融合核心的特征分析
Biochem Biophys Res Commun. 2015 May 8;460(3):633-8. doi: 10.1016/j.bbrc.2015.03.081. Epub 2015 Mar 21.
2
Molecular properties of a hemagglutinin purified from type A Clostridium botulinum.从A型肉毒梭菌中纯化的血凝素的分子特性
J Protein Chem. 1999 Jan;18(1):29-38. doi: 10.1023/a:1020691215056.
3
Mutational analysis of the oligomer assembly domain in the transmembrane subunit of the Rous sarcoma virus glycoprotein.劳氏肉瘤病毒糖蛋白跨膜亚基中寡聚体组装结构域的突变分析。
J Virol. 1997 Mar;71(3):2383-9. doi: 10.1128/JVI.71.3.2383-2389.1997.
4
Antibody cross-competition analysis of the human immunodeficiency virus type 1 gp120 exterior envelope glycoprotein.人类免疫缺陷病毒1型gp120外膜糖蛋白的抗体交叉竞争分析
J Virol. 1996 Mar;70(3):1863-72. doi: 10.1128/JVI.70.3.1863-1872.1996.
5
Correlation between fusogenicity of synthetic modified peptides corresponding to the NH2-terminal extremity of simian immunodeficiency virus gp32 and their mode of insertion into the lipid bilayer: an infrared spectroscopy study.猿猴免疫缺陷病毒gp32氨基末端对应合成修饰肽的融合活性与其插入脂质双层模式之间的相关性：一项红外光谱研究。
J Virol. 1994 Feb;68(2):1139-48. doi: 10.1128/JVI.68.2.1139-1148.1994.
6
Immunological evidence for interactions between the first, second, and fifth conserved domains of the gp120 surface glycoprotein of human immunodeficiency virus type 1.1型人类免疫缺陷病毒表面糖蛋白gp120第一、第二和第五保守结构域之间相互作用的免疫学证据。
J Virol. 1994 Nov;68(11):6836-47. doi: 10.1128/JVI.68.11.6836-6847.1994.
本文引用的文献
1
Orientation and structure of the NH2-terminal HIV-1 gp41 peptide in fused and aggregated liposomes.NH2末端HIV-1 gp41肽在融合和聚集脂质体中的取向和结构。
Biochim Biophys Acta. 1993 Jan 18;1145(1):124-33. doi: 10.1016/0005-2736(93)90389-h.
2
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.流感病毒血凝素膜糖蛋白在3埃分辨率下的结构
Nature. 1981 Jan 29;289(5796):366-73. doi: 10.1038/289366a0.
3
Infrared membrane spectroscopy.红外膜光谱法。
Mol Biol Biochem Biophys. 1981;31:270-332. doi: 10.1007/978-3-642-81537-9_6.
4
Resolution-enhanced Fourier transform infrared spectroscopy of enzymes.酶的分辨率增强傅里叶变换红外光谱法。
Methods Enzymol. 1986;130:290-311. doi: 10.1016/0076-6879(86)30015-6.
5
Examination of the secondary structure of proteins by deconvolved FTIR spectra.通过去卷积傅里叶变换红外光谱对蛋白质二级结构进行检测。
Biopolymers. 1986 Mar;25(3):469-87. doi: 10.1002/bip.360250307.
6
Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.1型人类免疫缺陷病毒包膜糖蛋白的功能区
Science. 1987 Sep 11;237(4820):1351-5. doi: 10.1126/science.3629244.
7
Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins.肽、多肽和蛋白质的振动光谱与构象
Adv Protein Chem. 1986;38:181-364. doi: 10.1016/s0065-3233(08)60528-8.
8
Structure and expression of the human and mouse T4 genes.人类和小鼠T4基因的结构与表达
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9155-9. doi: 10.1073/pnas.84.24.9155.
9
Attenuated total reflectance Fourier transform infrared studies of the interaction of melittin, two fragments of melittin, and delta-hemolysin with phosphatidylcholines.蜂毒肽、蜂毒肽的两个片段以及δ-溶血素与磷脂酰胆碱相互作用的衰减全反射傅里叶变换红外光谱研究
Biochemistry. 1987 Dec 15;26(25):8151-8. doi: 10.1021/bi00399a020.
10
HIV and HLA similarity.人类免疫缺陷病毒与人类白细胞抗原的相似性。
Nature. 1988 May 19;333(6170):215. doi: 10.1038/333215c0.
Zotero 插件