多氯联苯同系物3,3',4,4'-四氯联苯对小鼠胎儿7-乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性的诱导作用。

Induction of 7-ethoxyresorufin-O-deethylase (EROD) activity in mice foetuses by the PCB-congener 3,3',4,4'-tetrachlorobiphenyl.

Sinjari T, Törnwall U, Darnerud P O

Department of Toxicology, BMC, Uppsala, Sweden.

Xenobiotica. 1993 Feb;23(2):107-14. doi: 10.3109/00498259309059366.

Abstract

3,3',4,4'-Tetrachlorobiphenyl (TCB) induces liver microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity, and this P450 isoform, i.e. CYP 1, is involved in the metabolism of TCB. 2. TCB administered i.p. to pregnant mice (5-25 mg/kg) induced foetal as well as maternal hepatic EROD activity; in all cases the maternal induced activity was higher than the foetal activity. EROD activity of TCB-induced foetuses increased with developmental age, but decreased with the length of time after treatment. 3. Strain differences were seen; in control mice and TCB-treated dams NMRI > C57BL in EROD activity, but in transplacentally TCB-treated foetuses, C57BL > NMRI. EROD activity in non-pregnant control C57BL mice was higher than in pregnant ones, whereas no activity was seen in control foetuses. 4. Results indicate that TCB at a certain dose can induce foetal metabolism, but that earlier observed foetal accumulation at lower TCB doses is probably a consequence of transplacental transport of maternally-produced metabolites.

摘要

3,3',4,4'-四氯联苯（TCB）可诱导肝微粒体7-乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性，且这种细胞色素P450同工酶，即CYP 1，参与TCB的代谢。2. 对怀孕小鼠腹腔注射TCB（5 - 25毫克/千克）可诱导胎儿及母体肝脏的EROD活性；在所有情况下，母体诱导的活性均高于胎儿活性。TCB诱导的胎儿的EROD活性随发育年龄增加，但随治疗后时间的延长而降低。3. 观察到品系差异；在对照小鼠和经TCB处理的母鼠中，NMRI品系的EROD活性高于C57BL品系，但在经胎盘接受TCB处理的胎儿中，C57BL品系 > NMRI品系。未怀孕的对照C57BL小鼠的EROD活性高于怀孕小鼠，而对照胎儿中未观察到活性。4. 结果表明，一定剂量的TCB可诱导胎儿代谢，但早期观察到的较低剂量TCB时胎儿的蓄积可能是母体产生的代谢产物经胎盘转运的结果。