Xia Y, Feng L, Yoshimura T, Wilson C B
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
Am J Physiol. 1993 May;264(5 Pt 2):F774-80. doi: 10.1152/ajprenal.1993.264.5.F774.
The capacity of the lipopolysaccharide (LPS)-stimulated isolated erythrocyte-perfused rat kidney (IEPK) to produce monocyte chemoattractant protein-1 (MCP-1), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) mRNA was investigated. The IEPK was chosen to exclude the influence of circulating neutrophils and monocytes that can produce both these mediators when exposed to LPS. The control minimal LPS group (LPS < 10 pg/ml) showed a small increase in mRNA expression for MCP-1, IL-1 beta, and TNF-alpha in the cortex and medulla after 80 min of perfusion when compared with the unperfused left kidney in which no IL-1 beta or TNF-alpha mRNA and only minimal amounts of MCP-1 mRNA were detected. LPS stimulation (1 microgram/ml for 40 or 80 min) increased MCP-1, IL-1 beta, and TNF-alpha mRNA expression, which was found predominately in peritubular capillary endothelial cells by in situ hybridization. The changes were not due to a marked perturbation of LPS on renal hemodynamics. The renal vascular resistance (RVR) remained constant (40 min LPS exposure) or increased only slightly during the last 5-10 min (80 min LPS exposure) compared with a progressive increase in RVR of the minimal LPS group. The hemodynamic effects of LPS on the IEPK appear to counteract the gradual increase in RVR seen in the minimal LPS group.
研究了脂多糖（LPS）刺激的离体红细胞灌注大鼠肾脏（IEPK）产生单核细胞趋化蛋白-1（MCP-1）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）mRNA的能力。选择IEPK以排除循环中性粒细胞和单核细胞的影响，这些细胞在接触LPS时可产生这两种介质。与未灌注的左肾相比，对照最小LPS组（LPS<10 pg/ml）在灌注80分钟后，皮质和髓质中MCP-1、IL-1β和TNF-α的mRNA表达略有增加，未灌注的左肾未检测到IL-1β或TNF-αmRNA，仅检测到少量MCP-1 mRNA。LPS刺激（1μg/ml，40或80分钟）增加了MCP-1、IL-1β和TNF-α的mRNA表达，原位杂交发现其主要存在于肾小管周围毛细血管内皮细胞中。这些变化不是由于LPS对肾血流动力学的显著干扰所致。与最小LPS组肾血管阻力（RVR）的逐渐增加相比，肾血管阻力在LPS暴露40分钟时保持恒定或在最后5-10分钟（LPS暴露80分钟）仅略有增加。LPS对IEPK的血流动力学影响似乎抵消了最小LPS组中RVR的逐渐增加。
