Miranda G, Anglade P, Mahé M F, Erhardt G
Station de Recherches Laitières, INRA CRJ, Jouy-en-Josas, France.
Anim Genet. 1993 Feb;24(1):27-31. doi: 10.1111/j.1365-2052.1993.tb00915.x.
Analysis of elution profiles of enzymatic and CNBr digests of kappa-caseins C and E, and sequencing of most relevant peptides allowed the chemical characterization of both genetic variants. They differ from their B and A allelic counterparts by a single substitution, His97/Arg and Gly155/Ser, respectively. Electrophoretic behaviour of the investigated C and E variants was in good agreement with the observed amino acid replacements.
对κ-酪蛋白C和E的酶解及溴化氰消化产物的洗脱图谱进行分析，并对最相关的肽段进行测序，从而对这两种遗传变体进行化学表征。它们与其B和A等位基因对应物的区别分别在于单个取代，即His97/Arg和Gly155/Ser。所研究的C和E变体的电泳行为与观察到的氨基酸替换情况高度一致。
